| Title | Magnesium-pretreated periosteum for promoting bone-tendon healing after anterior cruciate ligament reconstruction |
| Authors | Wang, Jiali Xu, Jiankun Wang, Xinluan Sheng, Liyuan Zheng, Lizhen Song, Bin Wu, Ge Zhang, Ri Yao, Hao Zheng, Nianye Ong, Michael Tim Yun Yung, Patrick Shu-hang Qin, Ling |
| Affiliation | Sun Yat Sen Univ, Biomed Engn Sch, Guangzhou 510006, Peoples R China Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Musculoskeletal Res Lab, Dept Orthopaed & Traumatol, Hong Kong, Peoples R China Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Drug Translat Res Lab, Hong Kong, Peoples R China Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Translat Med R&D Ctr, Shenzhen, Peoples R China Peking Univ, Shenzhen Inst, Shenzhen 518057, Guangdong, Peoples R China Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Sports Med, Guangzhou 510006, Peoples R China City Univ Hong Kong, Dept Mech & Biomed Engn, Kowloon, Hong Kong, Peoples R China |
| Keywords | GROWTH-FACTOR GRAFT TUNNEL CELLS FAILURE TISSUE |
| Issue Date | Jan-2021 |
| Publisher | BIOMATERIALS |
| Abstract | Periosteum can improve tendon-bone healing when applied to wrap the tendon graft in both animal studies and clinical trials. As magnesium (Mg) ions can significantly elevate the levels of relevant cytokines involving in the osteogenic differentiation of periosteum-derived stem cells, the Mg-pretreated periosteum may be an innovative approach for enveloping the tendon graft. To test this hypothesis, we compared the effects of Mg-pretreated periosteum (M - P) and the stainless steel (SS)-pretreated periosteum (SS-P) in ACL reconstruction. We firstly found that the released Mg ions from the Mg implants were partially accumulated in periosteum, resulting in higher Mg/Ca ratio in the M P compared to the SS-P. Additionally, the M - P showed significantly higher expression levels of calcitonin gene-related peptide (CGRP) and periostin than the SS-P due to the decrease in Cathepsin K (CTSK). Elevation of CGRP and periostin was beneficial for the osteogenic differentiation of periosteum-derived stem cells. More importantly, we demonstrated that the M - P remarkably increased the formation of fibrocartilage at the interface between the periosteum and tendon. Collectively, M - P group demonstrated significantly prevented peri-tunnel bone loss, more osseous ingrowth into the tendon graft and higher maximum load to failure as compared to the SS-P group. In summary, our study warrants further investigations for translating the current proof-of-concept findings to optimize the delivery of CGRP, periostin, and cells as novel practical therapeutic strategy for enhancing tendon-bone interface healing in patients undergoing ACL reconstruction. |
| URI | http://hdl.handle.net/20.500.11897/604369 |
| ISSN | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2020.120576 |
| Indexed | SCI(E) |
| Appears in Collections: | 深圳研究生院待认领 |
