| Title | Silica nanoparticles induce unfolded protein reaction mediated apoptosis in spermatocyte cells |
| Authors | Ren, Lihua Liu, Jianhui Wei, Jialiu Du, Yefan Zou, Kaiyue Yan, Yongyang Wang, Zhihao Zhang, Linruo Zhang, Tong Lu, Hong Zhou, Xianqing Sun, Zhiwei |
| Affiliation | Peking Univ, Sch Nursing, Div Maternal & Child Nursing, Hlth Sci Ctr, 38 Xueyuan Rd, Beijing 100191, Peoples R China Capital Med Univ, Sch Publ Hlth, Dept Toxicol & Hyg Chem, 10 Xi Tou Tiao, Beijing 100069, Peoples R China Capital Med Univ, Beijing Key Lab Environm Toxicol, 10 Xi Tou Tiao, Beijing 100069, Peoples R China Chinese Acad Med Sci & Peking Union Med Coll, Fuwai Hosp, Natl Ctr Cardiovasc Dis, Dept Epidemiol,State Key Lab Cardiovasc Dis, 167 North Lishi Rd, Beijing 100037, Peoples R China |
| Keywords | ENDOPLASMIC-RETICULUM-STRESS INFLAMMATORY RESPONSE ER STRESS PATHWAYS ACID ASSOCIATION ACTIVATION PARTICLES DEATH |
| Issue Date | Jul-2020 |
| Publisher | TOXICOLOGY RESEARCH |
| Abstract | With increasing air pollution, silica nanoparticles (SiNPs), as a main inorganic member of PM2.5, have gained increasing attention to its reproductive toxicity. Most existing studies focused on the acute exposure, while data regarding the chronic effect of SiNPs on reproduction is limited. Therefore, this study was designed to evaluate the chronic toxicity of SiNPs on spermatocyte cells. The cells were continuously exposed to SiNPs for 1, 10, 20 and 30 generations at dose of 5 mu g/ml SiNPs for 24 h per generation after attachment. The results showed that with the increasing generations of the exposure, SiNPs decreased the viability of spermatocyte cells, induced apoptosis and increased the level of reactive oxygen species in spermatocyte cells. Moreover, SiNPs increased the protein expression of GRP-78, p-PERK, IRE1a, ATF6 and Cleaved caspase-3 in spermatocyte cells, suggesting that SiNPs improved unfolded protein response (UPR) and apoptosis. The present results indicated that the long-term and low-dose exposure to SiNPs could induce apoptosis by triggering ROS-mediated UPR in spermatocyte cells. |
| URI | http://hdl.handle.net/20.500.11897/602280 |
| ISSN | 2045-452X |
| DOI | 10.1093/toxres/tfaa036 |
| Indexed | SCI(E) |
| Appears in Collections: | 护理学院 |
