| Title | Spontaneous Atherosclerosis in Aged LCAT-Deficient Hamsters With Enhanced Oxidative Stress-Brief Report |
| Authors | Guo, Mengmeng Liu, Zongyu Xu, Yitong Ma, Ping Huang, Wei Gao, Mingming Wang, Yuhui Liu, George Xian, Xunde |
| Affiliation | Peking Univ, Inst Cardiovasc Sci, 38 Xueyuan Rd, Beijing 100191, Peoples R China Peking Univ, Key Lab Mol Cardiovasc Sci, Minist Educ, Beijing, Peoples R China Peking Univ, Sch Hlth Humanities, Beijing, Peoples R China Hebei Med Univ, Inst Basic Med, Lab Lipid Metab, Shijiazhuang, Hebei, Peoples R China |
| Keywords | LECITHIN-CHOLESTEROL ACYLTRANSFERASE LECITHINCHOLESTEROL ACYLTRANSFERASE SEVERE HYPERTRIGLYCERIDEMIA PLASMA MICE ESTERIFICATION DISEASE GENE DIET METABOLISM |
| Issue Date | Dec-2020 |
| Publisher | ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY |
| Abstract | Objective: LCAT (lecithin cholesterol acyltransferase) deficiency results in severe low HDL (high-density lipoprotein). Although whether LCAT is pro- or antiatherosclerosis was in debate in mouse studies, our previous study clearly shows that LCAT deficiency (LCAT(-/-)) in hamster accelerates atherosclerotic development on high-fat diet. However, unlike in hypercholesterolemia and hypertriglyceridemia, whether LCAT deficiency could lead to spontaneous atherosclerosis has not been studied yet in animal models. We, therefore, sought to investigate the atherosclerosis in LCAT(-/-) hamsters on standard laboratory diet and explore the potential underlying mechanisms. Approach and Results: Young (<8 months) and aged (>16 months) male and female wild-type and LCAT(-/-) hamsters on standard laboratory diet were used. Compared with age- and sex-matched wild-type hamsters, LCAT(-/-) hamsters showed a complete loss of plasma HDL and an increase in triglyceride by 2- to 8-fold at different stages of age. In aged LCAT(-/-) hamsters, the lesion areas at the aortic roots were approximate to 40x10(4) mu m(3) in males and 18x10(4) mu m(3) in females, respectively, which were consistent with the en face plaques observed in male (1.2%) and (1.5%) female groups, respectively. The results of plasma malondialdehyde measurement showed that malondialdehyde concentrations were markedly elevated to 54.4 mu mol/L in males and 30 mu mol/L in females, which are significantly associated with the atherosclerotic lesions. Conclusions: Our study demonstrates the development of spontaneous atherosclerotic lesions in aged male and female LCAT(-/-) hamsters with higher plasma oxidative lipid levels independent of plasma total cholesterol levels, further confirming the antiatherosclerotic role of LCAT. |
| URI | http://hdl.handle.net/20.500.11897/599701 |
| ISSN | 1079-5642 |
| DOI | 10.1161/ATVBAHA.120.315265 |
| Indexed | SCI(E) |
| Appears in Collections: | 基础医学院 分子心血管学教育部重点实验室 |
