| Title | Anti-ischemia/reperfusion injury effects of notoginsenoside R1 on small molecule metabolism in rat brain after ischemic stroke as visualized by MALDI-MS imaging |
| Authors | Zhu, Ting Wang, Lei Tian, Fang Zhao, Xin Pu, Xiao-Ping Sun, Gui-Bo Sun, Xiao-Bo |
| Affiliation | Peking Union Med Coll, Inst Med Plant Dev, Beijing Key Lab Innovat Drug Discovery Tradit Chi, Beijing 100193, Peoples R China Chinese Acad Med Sci, Beijing 100193, Peoples R China Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Key Lab Bioact Subst & Resources Utilizat Chinese, Minist Educ, Beijing 100193, Peoples R China Chinese Acad Med Sci, Key Lab New Drug Discovery Based Class Chinese Me, Beijing 100193, Peoples R China Harbin Univ Commerce, Harbin 150000, Heilongjiang, Peoples R China Peking Univ, Natl Key Res Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China |
| Keywords | CEREBRAL-ARTERY OCCLUSION MASS-SPECTROMETRY TISSUE-SECTIONS HIPPOCAMPAL CA1 GLOBAL BURDEN ACTIVATION PROTEINS NEUROPROTECTION NA-23 MICE |
| Issue Date | Sep-2020 |
| Publisher | BIOMEDICINE & PHARMACOTHERAPY |
| Abstract | Ischemic stroke is a syndrome of severe neurological responses that cause neuronal death, damage to the neurovascular unit and inflammation. Notoginsenoside R1 (NG-R1) is a neuroprotective drug that is commonly used to treat neurodegenerative and cerebrovascular diseases. However, its potential mechanisms on the regulation of small molecule metabolism in ischemic stroke are largely unknown. The aim of this study was to explore the potential mechanisms of NG-R1 on the regulation of small molecule metabolism after ischemic stroke. Here, we found that NG-R1 reduced infarct size and improved neurological deficits by ameliorating neuronal damage and inhibiting glial activation in MCAO/R rats. Furthermore, using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we clarified that NG-R1 regulated ATP metabolism, the tricarboxylic acid (TCA) cycle, the malate-aspartate shuffle, antioxidant activity, and the homeostasis of iron and phospholipids in the striatum and hippocampus of middle cerebral artery occlusion/reperfusion (MCAO/R) rats. In general, NG-R1 is a promising compound for brain protection from ischemic/reperfusion injury, possibly through the regulation of brain small molecule metabolism. |
| URI | http://hdl.handle.net/20.500.11897/591681 |
| ISSN | 0753-3322 |
| DOI | 10.1016/j.biopha.2020.110470 |
| Indexed | SCI(E) |
| Appears in Collections: | 天然药物与仿生药物国家重点实验室 |
