Title | LOX-1 RegulatesP. gingivalis-Induced Monocyte Migration and Adhesion to Human Umbilical Vein Endothelial Cells |
Authors | Li, Qian Liu, Jianru Liu, Wenyi Chu, Yi Zhong, Jinsheng Xie, Ying Lou, Xinzhe Ouyang, Xiangying |
Affiliation | Peking Univ, Dept Periodontol, Sch & Hosp Stomatol, Beijing, Peoples R China Peking Univ, Clin Div 1, Sch & Hosp Stomatol, Beijing, Peoples R China |
Keywords | LOW-DENSITY-LIPOPROTEIN NF-KAPPA-B PORPHYROMONAS-GINGIVALIS EXPRESSION RECEPTOR IDENTIFICATION INFLAMMATION MODULATION DISEASE |
Issue Date | 14-Jul-2020 |
Publisher | FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY |
Abstract | Porphyromonas gingivalis(P. gingivalis) is one of the main periodontal bacteria. This pathogen was reported to enhance monocyte migration and adhesion to endothelial cells in atherosclerosis. The scavenger receptor lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) plays a pivotal role in atherogenesis. The aim of this study was to investigate whether LOX-1 modulatesP. gingivalis-mediated monocyte migration and adhesion to endothelial cells and how it works. The results showed that the migration and adhesion of monocytic THP-1 cells to human umbilical vein endothelial cells (HUVECs) were significantly enhanced when HUVECs or THP-1 cells were challenged withP. gingivalis. Meanwhile, the expression level of LOX-1 in both HUVECs and THP-1 cells were also significantly increased byP. gingivalisstimulation. It is well known that ligand/receptor pairs monocyte chemoattractant protein-1 (MCP-1)/CC chemokine receptor 2 (CCR2), selectins/Integrins, and cell adhesion molecules (CAMs)/Integrins mediate monocyte migration and adhesion to endothelial cells. In this study, LOX-1 was demonstrated to be crucially involved inP. gingivalis-induced THP-1 cell migration and adhesion to HUVECs, by regulating expression of ligands MCP-1, intercellular adhesion molecule-1 (ICAM-1) and E-selectin in HUVECs and that of their receptors CCR2 and Integrin alpha M beta 2 in THP-1 cells. The nuclear factor-kappa B (NF-kappa B) signaling pathway was proved to be involved in this process. In conclusion, LOX-1 plays a crucial role inP.gingivalis-induced monocyte migration and adhesion to endothelial cells. This result implies LOX-1 may act as a bridge in linking periodontitis to atherosclerosis. |
URI | http://hdl.handle.net/20.500.11897/590857 |
ISSN | 2296-634X |
DOI | 10.3389/fcell.2020.00596 |
Indexed | SCI(E) |
Appears in Collections: | 口腔医院 |