| Title | The characterization of Mediator 12 and 13 as conditional positive gene regulators in Arabidopsis |
| Authors | Liu, Qikun Bischof, Sylvain Harris, C. Jake Zhong, Zhenhui Zhan, Lingyu Nguyen, Calvin Rashoff, Andrew Barshop, William D. Sun, Fei Feng, Suhua Potok, Magdalena Gallego-Bartolome, Javier Zhai, Jixian Wohlschlegel, James A. Carey, Michael F. Long, Jeffrey A. Jacobsen, Steven E. |
| Affiliation | Peking Univ, Sch Adv Agr Sci, Beijing 100871, Peoples R China Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA Univ Zurich, Dept Plant & Microbial Biol, Zollikerstr 107, CH-8008 Zurich, Switzerland Univ Zurich, Zurich Basel Plant Sci Ctr, Zollikerstr 107, CH-8008 Zurich, Switzerland Fujian Agr & Forestry Univ, Basic Forestry & Prote Ctr, Fuzhou 350002, Peoples R China Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA Southern Univ Sci & Technol, Inst Plant & Food Sci, Dept Biol, Shenzhen, Peoples R China Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90095 USA |
| Keywords | RNA-POLYMERASE V DNA METHYLATION CDK8 MODULE COMPLEX TRANSCRIPTION EXPRESSION LIGHT SUBUNITS PROTEINS DOMAIN |
| Issue Date | 3-Jun-2020 |
| Publisher | NATURE COMMUNICATIONS |
| Abstract | Mediator 12 (MED12) and MED13 are components of the Mediator multi-protein complex, that facilitates the initial steps of gene transcription. Here, in an Arabidopsis mutant screen, we identify MED12 and MED13 as positive gene regulators, both of which contribute broadly to morc1 de-repressed gene expression. Both MED12 and MED13 are preferentially required for the expression of genes depleted in active chromatin marks, a chromatin signature shared with morc1 re-activated loci. We further discover that MED12 tends to interact with genes that are responsive to environmental stimuli, including light and radiation. We demonstrate that light-induced transient gene expression depends on MED12, and is accompanied by a concomitant increase in MED12 enrichment during induction. In contrast, the steady-state expression level of these genes show little dependence on MED12, suggesting that MED12 is primarily required to aid the expression of genes in transition from less-active to more active states. Mediator is a multiprotein complex required to activate gene transcription by RNAPII. Here, the authors report that MED12 and MED13 are conditional positive regulators that facilitate the expression of genes depleted in active chromatin marks and the induction of gene expression in response to environmental stimuli in Arabidopsis. |
| URI | http://hdl.handle.net/20.500.11897/590191 |
| ISSN | 2041-1723 |
| DOI | 10.1038/s41467-020-16651-5 |
| Indexed | SCI(E) |
| Appears in Collections: | 现代农学院 |
