| Title | Circulating MicroRNA-423-3p Improves the Prediction of Coronary Artery Disease in a General Population - Six-Year Follow-up Results From the China-Cardiovascular Disease Study - |
| Authors | Wang, Xin Dong, Ying Fang, Tian Wang, Xiaoxia Chen, Lu Zheng, Congyi Kang, Yuting Jiang, Linlin You, Xin Gai, Shujie Wang, Zengwu Cao, Huiqing |
| Affiliation | Peking Union Med Coll & Chinese Acad Med Sci, Fuwai Hosp, Natl Ctr Cardiovasc Dis, Div Prevent Community Hlth, 15 Lin, Beijing 102308, Peoples R China Capital Med Univ, Chaoyang Hosp, Heart Ctr, Beijing, Peoples R China Capital Med Univ, Chaoyang Hosp, Beijing Key Lab Hypertens, Beijing, Peoples R China Peking Univ, Inst Mol Med, Beijing, Peoples R China Yanbian Univ, Dept Lab Med, Affiliated Hosp, Jilin, Peoples R China Chinese Acad Med Sci & Pecking Union Med Coll, Fuwai Hosp, Natl Ctr Cardiovasc Dis, State Key Lab Cardiovasc Dis, Beijing, Peoples R China |
| Keywords | HEART-DISEASE MYOCARDIAL-INFARCTION PREVALENCE BIOMARKERS MIR-221 DEATH SERUM |
| Issue Date | Jul-2020 |
| Publisher | CIRCULATION JOURNAL |
| Abstract | Background: Circulating microRNAs (miRNA) are potential prognostic biomarkers for cardiovascular disease. We aimed to identify serum miRNA as an effective predictor for coronary artery disease (CAD) events in a general population cohort. Methods and Results: Serum miRNAs associated with CAD were determined by small RNA sequencing and quantitative RT-PCR. Further, the predictive ability of identified serum miRNAs was measured in a general population of 2,812 people. As a main outcome measure, CAD events were collected for 6 years and included acute myocardial infarction and subsequent myocardial infarction. Out of the 48 miRNA candidates, 5 miRNAs (miR-10a-5p, miR-126-3p, miR-210-3p, miR-423-3p and miR-92a-3p) showed better reliability and repeatability in serum. Then, the association of serum levels of the 5 miRNAs with CAD was validated. Furthermore, miR-10a-5p and miR-423-3p, which showed better performance, were tested in the large cohort, with a median follow up of 6.0 years. In multivariable Cox regression analysis, only miR-423-3p (P for trend<0.001) was able to precisely predict CAD events. Moreover, the addition of circulating miR-423-3p with the traditional risk factors together markedly improved the various model performance measures, including the area under the operating characteristics curve (0.782 vs. 0.806), Akaike Information Criterion (965.845 vs. 943.113) and net reclassification improvement (19.18%). Conclusions: Circulating miR-423-3p can improve the prediction of primary CAD outcomes on the basis of a traditional risk factor model in general population. |
| URI | http://hdl.handle.net/20.500.11897/590153 |
| ISSN | 1346-9843 |
| DOI | 10.1253/circj.CJ-19-1181 |
| Indexed | SCI(E) |
| Appears in Collections: | 分子医学研究所 |
