| Title | Visualization of Ligand-Bound Ectodomain Assembly in the Full-Length Human IGF-1 Receptor by Cryo-EM Single-Particle Analysis |
| Authors | Zhang, Xi Yu, Daqi Sun, Jingchuan Wu, Yujie Gong, Junyuan Li, Xuemei Liu, Li Liu, Shan Liu, Jianbo Wu, Yulan Li, Dongyang Ma, Yinping Han, Xu Zhu, Yanan Wu, Zhaolong Wang, Yihua Ouyang, Qi Wang, Tao |
| Affiliation | Harbin Inst Technol, Sch Life Sci & Technol, Harbin 150001, Peoples R China Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Peoples R China Peking Univ, Sch Phys, State Key Lab Artificial Microstruct & Mesoscop P, Beijing 100871, Peoples R China Peking Univ, Inst Syst Biol, Shenzhen Bay Lab, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China Peking Univ, Sch Phys, Electron Microscopy Lab, Beijing 100871, Peoples R China Peking Univ, Comp Ctr, Beijing 100871, Peoples R China Peking Univ, Peking Tsinghua Ctr Life Sci, Sch Phys, Beijing 100871, Peoples R China Peking Univ, Ctr Quantitat Biol, Beijing 100871, Peoples R China Longhua Ctr Dis Control & Prevent, Joint Lab Infect Dis Prevent & Control, Hygien Sect, Shenzhen 518109, Peoples R China |
| Keywords | 1ST 3 DOMAINS INSULIN-RECEPTOR BINDING ACTIVATION RESOLUTION ANALOGS SYSTEM |
| Issue Date | 5-May-2020 |
| Publisher | STRUCTURE |
| Abstract | Tyrosine kinase receptor of insulin-like growth factor 1 receptor (IGF-1R) and insulin receptor (IR) bind to hormones, such as insulin, IGF-1, and IGF-2, and transduces the signals across the cell membrane, However, the complete structure of the receptor and the signal transduction mechanism remains unclear. Here, we report the cryo-EM structure of the ligand-bound ectodomain in the full-length human IGF-1 R. We reconstructed the IGF-1 R/insulin complex at 4.7 angstrom and the IGF-1 R/IGF-1 complex at 7.7 angstrom. Our structures reveal that only one insulin or one IGF-1 molecule binds to and activates the full-length human IGF-1R receptor. |
| URI | http://hdl.handle.net/20.500.11897/588799 |
| ISSN | 0969-2126 |
| DOI | 10.1016/j.str.2020.03.007 |
| Indexed | SCI(E) Scopus |
| Appears in Collections: | 物理学院 人工微结构和介观物理国家重点实验室 深圳研究生院待认领 生命科学学院 |
