| Title | Flufenamic Acid Inhibits Adipogenic Differentiation of Mesenchymal Stem Cells by Antagonizing the PI3K/AKT Signaling Pathway |
| Authors | Liu, Xuenan Zheng, Li Liu, Hao Zhu, Yuan Xia, Dandan Wang, Siyi Gu, Ranli Zhang, Ping Liu, Yunsong Zhou, Yongsheng |
| Affiliation | Peking Univ, Natl Lab Digital & Mat Technol Stomatol, Sch & Hosp Stomatol,Dept Prosthodont, Beijing Key Lab Digital Stomatol,Natl Clin Res Ct, 22 Zhongguancun South Ave, Beijing 100081, Peoples R China Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China |
| Keywords | ENHANCES OSTEOGENIC DIFFERENTIATION SUPPRESSES BONE LOSS ADIPOCYTE DIFFERENTIATION OBESITY ASPIRIN OSTEOPOROSIS MARROW MECHANISMS EXPRESSION |
| Issue Date | 16-Mar-2020 |
| Publisher | STEM CELLS INTERNATIONAL |
| Abstract | Objectives. Flufenamic acid (FFA) is a representative of the fenamic acids, an important group of NSAIDs. In the present study, we study the effects of FFA on adipogenesis of human mesenchymal stem cells (MSCs) and we explore the potential mechanism. Methods. To investigate the effects of FFA on adipogenic differentiation of hMSCs, human adipose-derived stem cells (hASCs) and human bone marrow mesenchymal stem cells (hBMMSCs), representative of hMSCs, were treated with FFA during adipogenic differentiation in vitro. The effects of FFA in vivo were evaluated using a heterotopic adipose formation assay in nude mice as well as ovariectomized (OVX) and aged mice. To explore the mechanism of FFA, Western blot was used to determine activation of the PI3K/AKT signaling pathway. Results. Our results demonstrate that, at certain concentrations, FFA inhibited adipogenesis of human MSCs both in vitro and in vivo. Mechanistically, FFA inhibited adipogenesis of human MSCs by inhibiting the PI3K/AKT pathway. Conclusions. The present study indicated that FFA could be used to inhibit adipogenesis of human MSCs in tissue engineering and diseases related to excessive adipogenic differentiation of MSCs. |
| URI | http://hdl.handle.net/20.500.11897/587529 |
| ISSN | 1687-966X |
| DOI | 10.1155/2020/1540905 |
| Indexed | SCI(E) |
| Appears in Collections: | 口腔医院 工学院 |
