| Title | Fourier Transform Infrared Spectroscopy Monitoring of Dihydroartemisinin-Induced Growth Inhibition in Ovarian Cancer Cells and Normal Ovarian Surface Epithelial Cells |
| Authors | Li, Lei Wu, Jinguang Weng, Shifu Yang, Limin Wang, Huizi Xu, Yizhuang Shen, Keng |
| Affiliation | Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Obstet & Gynecol, 1 Shuai Fu Yuan, Beijing 100730, Peoples R China Peking Univ, Coll Chem & Mol Engn, State Key Lab Rare Earth Mat Chem & Applicat, Beijing Natl Lab Mol Sci, 202 Chengfu Rd, Beijing 100871, Peoples R China Peking Univ, Sch Phys, Inst Heavy Ion Phys, State Key Lab Nucl Phys & Technol, Beijing 100871, Peoples R China Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Med Sci Res Ctr, Beijing 100730, Peoples R China |
| Keywords | FTIR SPECTROSCOPY HL60 CELLS APOPTOSIS ARTEMISININ DRUG DEATH |
| Issue Date | 2020 |
| Publisher | CANCER MANAGEMENT AND RESEARCH |
| Abstract | Purpose: Ovarian cancer is the most lethal of gynecological malignancies. Dihydroartemisinin (DHA), a derivative of artemisinin (ARS), has profound effects against human tumors. The aim of this study was to provide a convenient, cost-efficient technique, Fourier transform infrared (FTIR) spectroscopy, to monitor and evaluate responses to DHA-induced growth inhibition of ovarian cancer cells. Methods: Cell growth and viability and the 50% inhibitory concentration (IC50) of DHA were assessed by the MTT assay. FTIR spectroscopy was used to monitor cells following DHA treatment, and data were analyzed by OMNIC 8.0 software. Results: DHA can decrease the viability of ovarian cancer cells and normal cells, but cancer cells were more sensitive to this drug than normal cells. Spectral differences were observed between cells with or without DHA treatment. In particular, an increase in the amount of lipids and nucleic acids was observed. The band intensity ratio of 1454/1400, and the intensity of the band 1741 cm(-1) increased, indicating stronger absorption after DHA treatment. Moreover, the differences were larger for the cell lines that were more sensitive to DHA. Conclusion: The spectral features provided information about important molecular characteristics of the cells in response to chemicals. These findings demonstrated the possible use of FTIR spectroscopy to evaluate DHA-induced growth inhibition effects in ovarian cancer cells and provided a promising new tool for monitoring cell growth and the effects of antitumor drugs in the clinic in the future. |
| URI | http://hdl.handle.net/20.500.11897/585615 |
| ISSN | 1179-1322 |
| DOI | 10.2147/CMAR.S240285 |
| Indexed | SCI(E) Scopus |
| Appears in Collections: | å å¦ä¸ å å å·¥ç¨ å¦é ¢ 稀土材料化学与应用国家重点实验室 物理学院 核物理与核技术国家重点实验室 |
