| Title | Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis |
| Authors | Yun, Juanli Zheng, Xiaowei Xu, Peng Zheng, Xu Xu, Jingyue Cao, Chen Fu, Yusi Xu, Bingxue Dai, Xin Wang, Yi Liu, Hongtao Yi, Qiaolian Zhu, Yaxin Wang, Jian Wang, Li Dong, Zhiyang Huang, Li Huang, Yanyi Du, Wenbin |
| Affiliation | Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China Peking Univ, Biomed Pioneering Innovat Ctr BIOPIC, Beijing Adv Innovat Ctr Genom ICG,Sch Life Sci, Peking Tsinghua Ctr Life Sci,Coll Engn, Beijing 100871, Peoples R China Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China Univ Calif San Francisco, Bioengn & Therapeut Sci, San Francisco, CA 94158 USA Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08540 USA |
| Keywords | HIGH-THROUGHPUT AMPLIFICATION TECHNOLOGIES MICROBES REVEALS GENOMES DISPLAY |
| Issue Date | Mar-2020 |
| Publisher | SMALL |
| Abstract | Single-cell analysis offers unprecedented resolution for the investigation of cellular heterogeneity and the capture of rare cells from large populations. Here, described is a simple method named interfacial nanoinjection (INJ), which can miniaturize various single-cell assays to be performed in nanoliter water-in-oil droplets on standard microwell plates. The INJ droplet handler can adjust droplet volumes for multistep reactions on demand with high precision and excellent monodispersity, and consequently enables a wide range of single-cell assays. Importantly, INJ can be coupled with fluorescence-activated cell sorting (FACS), which is currently the most effective and accurate single-cell sorting and isolation method. FACS-INJ pipelines for high-throughput plate well-based single-cell analyses, including single-cell proliferation, drug-resistance testing, polymerase chain reaction (PCR), reverse-transcription PCR, and whole-genome sequencing are introduced. This FACS-INJ pipeline is compatible with a wide range of samples and can be extended to various single-cell analysis applications in microbiology, cell biology, and biomedical diagnostics. |
| URI | http://hdl.handle.net/20.500.11897/584639 |
| ISSN | 1613-6810 |
| DOI | 10.1002/smll.201903739 |
| Indexed | SCI(E) Scopus EI |
| Appears in Collections: | 工学院 |
