| Title | Selective tracking of ovarian-cancer-specific -glutamyltranspeptidase using a ratiometric two-photon fluorescent probe |
| Authors | Shi, Ben Zhang, Zhenyu Jin, Qingqing Wang, Zhiqiang Tang, Jie Xu, Ge Zhu, Tianli Gong, Xueqing Tang, Xinjing Zhao, Chunchang |
| Affiliation | East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China. East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, Shanghai 200237, Peoples R China. Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China. East China Univ Sci & Technol, Sch Chem & Mol Engn, Ctr Computat Chem, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China. East China Univ Sci & Technol, Sch Chem & Mol Engn, Res Inst Ind Catalysis, 130 Meilong Rd, Shanghai 200237, Peoples R China. East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China. Zhao, CC (reprint author), East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, Shanghai 200237, Peoples R China. Tang, XJ (reprint author), Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China. |
| Keywords | GAMMA-GLUTAMYL-TRANSPEPTIDASE IN-VIVO TURN-ON HUMAN SERUM MITOCHONDRIA TRANSFERASE EXPRESSION STRATEGIES LYSOSOME CYSTEINE |
| Issue Date | 2018 |
| Publisher | JOURNAL OF MATERIALS CHEMISTRY B |
| Citation | JOURNAL OF MATERIALS CHEMISTRY B. 2018, 6(45), 7439-7443. |
| Abstract | Real-time tracking of GGT enzymatic activity in human ovarian cancer cells is a reliable method for accurate prediction of cancer diagnosis and management. Here, we report the two-photon ratiometric tracking of GGT activity in cancer cells based on a probe with switchable Forster resonance energy transfer properties. In the absence of GGT, the designed probe showed two well-resolved emission bands at 461 and 610 nm, corresponding to the 7-hydroxycoumarin donor and BODIPY acceptor, respectively. In contrast, GGT catalyzed cascade reactions including cleavage of the -glutamyl group and subsequent aromatic hydrocarbon transfer from the S to N atom increased the distance between the two chromophores, thus decreasing the FRET efficiency, with the recovery of the donor fluorescence at 461 nm. By exploiting this enzyme-triggered ratiometric measurement, successful differentiation of ovarian cancer cells from normal cells with this probe was realized by two-photon fluorescence confocal microscopy. |
| URI | http://hdl.handle.net/20.500.11897/570777 |
| ISSN | 2050-750X |
| DOI | 10.1039/c8tb01735b |
| Indexed | SCI(E) |
| Appears in Collections: | 药学院 天然药物与仿生药物国家重点实验室 |
