| Title | The Simplified Carbapenem Inactivation Method (sCIM) for Simple and Accurate Detection of Carbapenemase-Producing Gram-Negative Bacilli |
| Authors | Jing, Xiaopeng Zhou, Huan Min, Xiaochun Zhang, Xing Yang, Qing Du, Shuaixian Li, Yirong Yu, Fangyou Jia, Min Zhan, Yu Zeng, Yi Yang, Bo Pan, Yunjun Lu, Binghuai Liu, Rong Zeng, Ji |
| Affiliation | Huazhong Univ Sci & Technol, Wuhan Hosp 4, Puai Hosp, Dept Clin Lab,Tongii Med Coll, Wuhan, Hubei, Peoples R China. Huazhong Univ Sci & Technol, Minist Educ China Neurol Disorders, Dept Pathophysiol, Tongji Med Coll,Sch Basic Med,Key Lab, Wuhan, Hubei, Peoples R China. Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Clin Lab, Hangzhou, Zhejiang, Peoples R China. Huazhong Univ Sci & Technol, Union Hosp, Dept Clin Lab, Wuhan, Hubei, Peoples R China. Wuhan Univ, Zhongnan Hosp, Dept Clin Lab, Wuhan, Hubei, Peoples R China. Tongji Univ, Sch Med, Shanghai Pulm Hosp, Dept Clin Lab, Shanghai, Peoples R China. Wuhan First Hosp, Dept Clin Lab, Wuhan, Hubei, Peoples R China. Cent Hosp Wuhan, Dept Clin Lab, Wuhan, Hubei, Peoples R China. Hubei Univ Med, Renmin Hosp, Dept Clin Lab, Shiyan, Peoples R China. Peking Univ, Civil Aviat Sch Clin Med, Civil Aviat Gen Hosp, Dept Lab Med, Beijing, Peoples R China. |
| Keywords | carbapenemase modified carbapenem inactivation method gram-negative bacilli Enterobacteriaceae simplified carbapenem inactivation method KLEBSIELLA-PNEUMONIAE ENTEROBACTERIACEAE |
| Issue Date | 2018 |
| Publisher | FRONTIERS IN MICROBIOLOGY |
| Citation | FRONTIERS IN MICROBIOLOGY. 2018, 9. |
| Abstract | This study reports the simplified carbapenem inactivation method (sCIM) to detect carbapenemase-producing gram-negative bacilli in a simple and accurate manner. This method is based on the modified carbapenem inactivation method (mCIM) with the improvement of experimental procedures. Instead of incubating the antibiotic disk in the organism culture media, the organism to be tested was smeared directly onto the antibiotic disk in the sCIM. For evaluating the sensitivity and specificity of the method, a total of 196 Enterobacteriaceae, 73 Acinetobacter baumannii, and 158 Pseudomonas aeruginosa isolates were collected. Polymerase chain reaction (PCR) was used to detect the carbapenemase genes. Phenotypic evaluations were performed using both the sCIM and the mCIM. PCR results showed that, of the 196 Enterobacteriaceae strains, 147 expressed the carbapenemase genes bla(KPC-2 )(58.5%), bla(IMP-4) (21.8%), bla(IMP-2) (2.0%), bla(VIM-1) (6.1%), bla(NDM-1) (10.2%), and bla(OXA-48) (1.4%). sCIM results had high concordance with PCR results (99.5%) and mCIM results (100%) with the exception of one Klebsiella pneumoniae strain, which had an minimal inhibitory concentration (MIC) for imipenem of 0.25 mg/L. PCR demonstrated that 53 of the 73 A. baumannii isolates expressed the carbapenemase genes bla(OXA-23) (98.1%) and bla(VIM-2 )(1.8%). sCIM and PCR results corresponded but all A. baumannii isolates were carbapenemase negative by the mCIM. PCR demonstrated that 25 of the 158 P aeruginosa isolates expressed carbapenemase genes bla(VIM-1) (52%), bla(VIM-2) (8%), bla(VIM-44) (36%), and bla(IMP-4) (4%). sCIM results had high concordance with PCR results (100%) and the mCIM results (99.4%) with the exception of one P aeruginosa isolate that expressed the bla(VIM-4) gene. The sCIM offers specificity and sensitivity comparable to PCR but has the advantage of being more user-friendly. This method is suitable for routine use in most clinical microbiology laboratories for the detection of carbapenemase-producing gram-negative bacilli. |
| URI | http://hdl.handle.net/20.500.11897/568240 |
| ISSN | 1664-302X |
| DOI | 10.3389/fmicb.2018.02391 |
| Indexed | SCI(E) Medline |
| Appears in Collections: | 北京民用航空总医院 |
