| Title | The application of BMP-12-overexpressing mesenchymal stem cells loaded 3D-printed PLGA scaffolds in rabbit rotator cuff repair |
| Authors | Chen, Peng Cui, Lei Chen, Guofei You, Tian Li, Wei Zuo, Jianwei Wang, Chen Zhang, Wentao Jiang, Changqing |
| Affiliation | Peking Univ, Shenzhen Hosp, Dept Sports Med, Shenzhen 518000, Peoples R China Anhui Med Univ, Clin Med Coll 1, Hefei 230000, Anhui, Peoples R China Shenzhen Guangming New Dist Peoples Hosp, Dept Orthoped, Shenzhen 518000, Peoples R China |
| Keywords | BM-MSCs Rotator cuff repair 3D print PLGA scaffolds |
| Issue Date | 2019 |
| Publisher | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES |
| Abstract | This study investigates if the application of bone marrow-derived mesenchymal stem cells (BM-MSCs) loaded 3D-printed scaffolds could improve rotator cuff repair. The polylactic-co-glycolic acid (PLGA) scaffolds were fabricated by 3D print technology. Rabbit BM-MSCs were transfected with a recombinant adenovirus encoding bone morphogenic protein 12 (BMP-12). The effect of BM-MSCs loaded PLGA scaffolds on tendon-bone healing was assessed by biomechanical testing and histological analysis in a rabbit rotator cuff repair model. We found that the PLGA scaffolds had good biocompatible and biodegradable property. Overexpression of BMP-12 increased the mRNA and protein expression of tenogenic genes in BM-MSCs cultured with DMEM medium and seeded in PLGA scaffolds. When BMP-12-overexpressing BM-MSCs-loaded PLGA scaffolds were implanted into the injured rabbit supraspinatus tendon-bone junctions, the tendon-bone healing was improved. Our results suggest that application of BMP-12 overexpressing BM-MSCs loaded 3D-printed PLGA scaffolds promote the healing of tendon-bone interface, improve collagen organization and increase fibrocartilage in the rabbit rotor cuff repair. Rotator cuff regeneration achieved by BMP-12-overexpressing BM-MSCs-loaded PLGA scaffolds may represent a novel approach for the management of rotator cuff defect. (C) 2019 Elsevier B.V. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/553531 |
| ISSN | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2019.07.041 |
| Indexed | SCI(E) |
| Appears in Collections: | 深圳医院 |
