| Title | Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg(-/-) mice |
| Authors | Du, Yaqin Wang, Ting Xu, Jun Zhao, Chaoran Li, Haibo Fu, Yao Xu, Yaxing Xie, Liangfu Zhao, Jingru Yang, Weifeng Yin, Ming Wen, Jinhua Deng, Hongkui |
| Affiliation | Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Dept Cell Biol,Sch Basic Med Sci, Beijing 100191, Peoples R China Peking Univ, Coll Life Sci, Peking Univ Tsinghua Univ Natl Inst Biol Sci Join, Beijing 100871, Peoples R China Peking Univ, MOE Key Lab Cell Proliferat & Differentiat, Coll Life Sci, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China Beijing Vitalstar Biotechnol, Beijing 100012, Peoples R China |
| Keywords | extended pluripotent stem cell NOD-scid Il2rg- -mice embryonic and extraembryonic lineages chemical reprogramming |
| Issue Date | 2019 |
| Publisher | PROTEIN & CELL |
| Abstract | Recently we have established a new culture condition enabling the derivation of extended pluripotent stem (EPS) cells, which, compared to conventional pluripotent stem cells, possess superior developmental potential and germline competence. However, it remains unclear whether this condition permits derivation of EPS cells from mouse strains that are refractory or non-permissive to pluripotent cell establishment. Here, we show that EPS cells can be robustly generated from non-permissive NOD-scid Il2rg(-/-) mice through de novo derivation from blastocysts. Furthermore, these cells can also be efficiently generated by chemical reprogramming from embryonic NOD-scid Il2rg(-/-) fibroblasts. NOD-scid Il2rg(-/-) EPS cells can be expanded for more than 20 passages with genomic stability and can be genetically modified through gene targeting. Notably, these cells contribute to both embryonic and extraembryonic lineages in vivo. More importantly, they can produce chimeras and integrate into the E13.5 genital ridge. Our study demonstrates the feasibility of generating EPS cells from refractory mouse strains, which could potentially be a general strategy for deriving mouse pluripotent cells. The generation of NOD-scid Il2rg(-/-) EPS cell lines permits sophisticated genetic modification in NOD-scid Il2rg(-/-) mice, which may greatly advance the optimization of humanized mouse models for biomedical applications. |
| URI | http://hdl.handle.net/20.500.11897/552244 |
| ISSN | 1674-800X |
| DOI | 10.1007/s13238-018-0558-z |
| Indexed | SCI(E) |
| Appears in Collections: | 医学部待认领 生命科学学院 细胞增殖分化调控机理研究教育部重点实验室 |
