| Title | Analysis of the chromatin binding affinity of retrotransposases reveals novel roles in diploid and tetraploid cotton |
| Authors | Lin, Jing Cai, Ying Huang, Gai Yang, Yan Li, Yang Wang, Kun Wu, Zhiguo |
| Affiliation | Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China Wuhan Univ, Inst Adv Studies, Wuhan 430072, Hubei, Peoples R China |
| Issue Date | 2019 |
| Publisher | JOURNAL OF INTEGRATIVE PLANT BIOLOGY |
| Abstract | LTR-retrotransposable elements are major components of diploid (Gossypium arboreum) and tetraploid (Gossypium hirsutum) cotton genomes that have undergone dramatic increases in copy number during the course of evolution. However, little is known about the biological functions of LTR-retrotransposable elements in cotton. Here, we show that a copia-like LTR-retrotransposable element has maintained considerable activity in both G. arboreum and G. hirsutum. We identified two functional domains of the retrotransposon and analyzed their expression levels in various cotton tissues, including leaves, ovules, and germinating seeds. ChIP-qPCR (chromatin immunoprecipitation followed by quantitative PCR), using a copia-specific antibody, established that copia-like proteins primarily bind to the first exons of several protein-coding genes in cotton cells. This finding suggests that retrotransposons play a novel, important role in regulating the transcriptional activities of protein-coding genes with various biological activities. |
| URI | http://hdl.handle.net/20.500.11897/552222 |
| ISSN | 1672-9072 |
| DOI | 10.1111/jipb.12740 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 生命科学学院 |
