| Title | Human omental adipose-derived stem cells from donors with different body mass index had similar effects on proliferation and migration of endometrial cancer cells in vitro |
| Authors | Li, Mingxia Li, Xiaoping Zhao, Lijun Zhou, Jingyi Cheng, Yuan Wang, Jianliu Wei, Lihui |
| Affiliation | Peking Univ, Peoples Hosp, Dept Gynecol & Obstet, 11 Xizhimen South St, Beijing 100044, Peoples R China |
| Keywords | adipose-derived stem cell body mass index endometrial cancer obesity |
| Issue Date | 2019 |
| Publisher | JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH |
| Abstract | Aim This study aimed to confirm whether human omental adipose-derived stem cells (O-ASC) from donors with varying body mass index (BMI, calculated in kg/m(2)) exert different effects on proliferation and migration of endometrial cancer (EC) cells. Methods Omental adipose-derived stem cells were isolated from the omental adipose tissues of eight patients who were diagnosed with EC aged from 35 to 56 years. According to the patients' BMI, the O-ASC was divided into two groups: obesity group (BMI >= 30) and normal group (18.5 < BMI <= 24.9). A broad spectrum cytokine antibody array was used to measure 62 paracrine cytokines secreted by all the O-ASC. MTS assays, direct and indirect co-culture assays were used to assess the effects of O-ASC on proliferation and migration of Hec-1A (estrogen receptor-/progesterone receptor-) and Ishikawa (estrogen receptor+/progesterone receptor+) EC cells. Results Eight samples of O-ASC were successfully isolated including four samples in obesity group and four in normal group. All the O-ASC displayed typical characteristics of mesenchymal stem cells and possessed similar secretory functions as 26 cytokines were identified in condition medium of O-ASC based on cytokine antibody array. The proliferation of Ishikawa cells was gently stimulated by O-ASC from the two groups (P < 0.05) whereas without any effects on Hec-1A cells. Both horizontal and vertical migrations of EC cells were promoted by O-ASC (P < 0.01). However, there were no statistical differences between the two groups. Conclusion Human O-ASC could influence the proliferation and migration of EC cells in vitro but the effects were not modified by donors' BMI. |
| URI | http://hdl.handle.net/20.500.11897/551126 |
| ISSN | 1341-8076 |
| DOI | 10.1111/jog.13820 |
| Indexed | SCI(E) |
| Appears in Collections: | 人民医院 |
