| Title | Automated quantification of Ki-67 index associates with pathologic grade of pulmonary neuroendocrine tumors |
| Authors | Wang, Hai-Yue Li, Zhong-Wu Sun, Wei Yang, Xin Zhou, Li-Xin Huang, Xiao-Zheng Jia, Ling Lin, Dong-Mei |
| Affiliation | Peking Univ Canc Hosp & Inst, Dept Pathol, Minist Educ, Key Lab Carcinogenesis & Translat Res, Beijing 100142, Peoples R China |
| Keywords | Antigen Ki-67 Computer-assisted numerical analysis Neuroendocrine tumor Prognostic factor |
| Issue Date | 2019 |
| Publisher | CHINESE MEDICAL JOURNAL |
| Abstract | Background: Classification of the pulmonary neuroendocrine tumor (pNET) categories is a step-wise process identified by the presence of necrosis and number of mitoses per 2 mm(2). In neuroendocrine tumor pathology, Ki-67 was first described as a prognostic factor in the pancreas and incorporated into the grading system of digestive tract neuroendocrine neoplasms in the 2010 WHO classification. However, the significance of Ki-67 in pNETs was still a controversial issue. This study was to investigate the potentially diagnostic value of Ki-67 in pNETs. Methods: We retrieved 159 surgical specimens of pNETs, including 35 typical carcinoids (TCs), 2 atypical carcinoid (ACs), 28 large-cell neuroendocrine carcinomas (LCNECs), 94 small-cell lung cancers (SCLCs). Manual conventional method (MCM) and computer-assisted image analysis method (CIAM) were used to calculate the Ki-67 proliferative index. In CLAM, 6 equivalent fields (500 x 500 mu m) at 10 x magnification were manually annotated for digital image analysis. Results: The Ki-67 index among the 4 groups with ranges of 0.38% to 12.66% for TC, 4.34% to 29.48% for AC, 30.67% to 93.74% for LCNEC, and 40.71% to 96.87% for SCLC. The cutoff value of Ki-67 index to distinguish low grade with high grade was 30.07%. For the univariate survival analyses in pNETs, both the overall survival and progression-free survival correlated with Ki-67 index. In addition, the Ki-67 index performed by CIAM was proved to be of great positive correlation with MCM. Conclusions: Ki-67 index counted by CIAM is a reliable method and can be a useful adjunct to classify the low- and high-grade NETs. |
| URI | http://hdl.handle.net/20.500.11897/550025 |
| ISSN | 0366-6999 |
| DOI | 10.1097/CM9.0000000000000109 |
| Indexed | SCI(E) |
| Appears in Collections: | 北京肿瘤医院 |
