| Title | The SOS2-SCaBP8 Complex Generates and Fine-Tunes an AtANN4-Dependent Calcium Signature under Salt Stress |
| Authors | Ma, Liang Ye, Jiamin Yang, Yongqing Lin, Huixin Yue, Lili Luo, Jin Long, Yu Fu, Haiqi Liu, Xiangning Zhang, Yulin Wang, Yi Chen, Liangyi Kudla, Joerg Wang, Youjun Han, Shengcheng Song, Chun-Peng Guo, Yan |
| Affiliation | China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China Peking Univ, Inst Mol Med, Lab Cell Secret & Metab, Beijing 100871, Peoples R China Univ Munster, Inst Biol & Biotechnol Pflanzen, Schlosspl 4, D-48149 Munster, Germany Beijing Normal Univ, Coll Life Sci, Beijing Key Lab Gene Resource & Mol Dev, Beijing, Peoples R China Henan Univ, Collaborat Innovat Ctr Crop Stress Biol, Inst Plant Stress Biol, Kaifeng 475001, Henan, Peoples R China |
| Issue Date | 2019 |
| Publisher | DEVELOPMENTAL CELL |
| Abstract | Calcium signals act as universal second messengers that trigger many cellular processes in animals and plants, but how specific calcium signals are generated is not well understood. In this study, we determined that AtANN4, a putative calcium-permeable transporter, and its interacting proteins, SCaBP8 and SOS2, generate a calcium signal under salt stress, which initially activates the SOS pathway, a conserved mechanism that modulates ion homeostasis in plants under salt stress. After activation, SCaBP8 promotes the interaction of protein kinase SOS2 with AtANN4, which enhances its phosphorylation by SOS2. This phosphorylation of AtANN4 further increases its interaction with SCaBP8. Both the interaction with and phosphorylation of AtANN4 repress its activity and alter calcium transients and signatures in HEK cells and plants. Our results reveal how downstream targets are required to create a specific calcium signal via a negative feedback regulatory loop, thereby enhancing our understanding of the regulation of calcium signaling. |
| URI | http://hdl.handle.net/20.500.11897/549975 |
| ISSN | 1534-5807 |
| DOI | 10.1016/j.devcel.2019.02.010 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 分子医学研究所 |
