| Title | Naked Oat (Avena nuda L.) Oligopeptides: Immunomodulatory Effects on Innate and Adaptive Immunity in Mice via Cytokine Secretion, Antibody Production, and Th Cells Stimulation |
| Authors | Mao, Ruixue Wu, Lan Zhu, Na Liu, Xinran Liu, Rui Li, Yong |
| Affiliation | Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Beijing 100191, Peoples R China Inner Mongolia Adm Market Regulat, Hohhot 010010, Peoples R China |
| Keywords | oat immunomodulatory oligopeptide cell-mediated immunity humoral immunity macrophage phagocytosis NK cell activity T cell subpopulation cytokine immunoglobulin |
| Issue Date | 2019 |
| Publisher | NUTRIENTS |
| Abstract | The study aimed to investigate the immunomodulatory activity of oligopeptides derived from oat (Avena nuda L.) (OOPs). Healthy female BALB/c mice were randomly assigned to five groups, given deionized water (control) and 0.25, 0.5, 1.0, and 2.0 g/kg body weight (BW) of OOPs daily by intragastric administration. Seven assays were performed to determine the immunomodulatory effects of OOPs on immune organ ratios, cellular and humoral immune responses, macrophage phagocytosis, and natural killer (NK) cell activity. Spleen T lymphocyte subpopulations (by flow cytometry), serum cytokine and immunoglobulin levels (by multiplex sandwich immunoassays) were determined to evaluate how OOPs affected the immune system. Our results showed that OOPs could significantly improve innate and adaptive immune responses in mice through the enhancement of cell-mediated and humoral immunity, macrophage phagocytosis capacity, and NK cell activity. We concluded that the immunomodulatory effects might be attributed to increased T and Th cell percentages, serum interferon (IFN)-, interleukin (IL)-1 , IL-2, IL-6, IL-10, IL-12, tumor necrosis factor (TNF)- , and granulocyte-macrophage colony-stimulating factor (GM-CSF) secretions as well as immunoglobulin (Ig) A, IgG, and IgM productions. These results indicate that dietary OOPs could be considered as promising immunomodulators with dosages ranging from 0.25 to 2.0 g/kg BW. |
| URI | http://hdl.handle.net/20.500.11897/549325 |
| ISSN | 2072-6643 |
| DOI | 10.3390/nu11040927 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 公共卫生学院 |
