| Title | Function of complement factor H and imaging of small molecules by MALDI-MSI in a methamphetamine behavioral sensitization model |
| Authors | Xu, Jiamin Zhang, Zhilin Liu, Runzhe Sun, Yi Liu, Huihui Nie, Zongxiu Zhao, Xin Pu, Xiaoping |
| Affiliation | Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China Chinese Acad Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China Beijing Ctr Mass Spectrometry, Beijing 100190, Peoples R China |
| Keywords | Methamphetamine Behavioral sensitization model Complement factor H C1q Matrix-assisted laser desorption time of flight ionization mass spectrometry imaging (MALDI-TOF-MSI) Metabolites |
| Issue Date | 2019 |
| Publisher | BEHAVIOURAL BRAIN RESEARCH |
| Abstract | Background: At present, the harm of new-type drug, methamphetamine (METH), has gradually exceeded that of the traditional opioid drugs, and METH abuse has become a serious public health and social problem. In our previous study, complement factor H (CFH) was found to be upregulated in the sera of METH-addicted patients and rats and in certain brain regions in the rats. Methods: We used ELISA and immunofluorescence to confirm the changes in CFH in the serum and hippocampus of a METH behavioral sensitization mouse model, and C1q expression was also detected by immunofluorescence in the hippocampus. We aimed to elucidate the involvement of CFH and C1q in the mechanism of METH addiction. We also detected the distribution of various small molecules by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) in select brain regions: the nucleus accumbens, the hippo campus and the ventral tegmental area. Results: The expression of CFH was upregulated in the serum and hippocampus of METH behavioral sensitization model mice, consistent with our previous research on conditioned place preference rats. In contrast, C1q decreased dramatically in the mossy fibers of the hippocampus. The results of small-molecule imaging by MALDI-MSI showed that the levels of K+, antioxidants, neurotransmitters, and ATP metabolism-related molecules were altered in different regions. Conclusions: These results indicate the involvement of the complement system in the mechanism of METH addiction and validate the presence of oxidative stress, energy metabolism changes during addiction. This suggests the utility of further investigation into the above aspects. |
| URI | http://hdl.handle.net/20.500.11897/548239 |
| ISSN | 0166-4328 |
| DOI | 10.1016/j.bbr.2019.02.002 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 天然药物与仿生药物国家重点实验室 药学院 |
