| Title | Long Noncoding RNA SBF2-AS1 Is Critical for Tumorigenesis of Early-Stage Lung Adenocarcinoma |
| Authors | Chen, Rui Xia, Wenjia Wang, Siwei Xu, Youtao Ma, Zhifei Xu, Weizhang Zhang, Erbao Wang, Jie Fang, Tian Zhang, Quan'an Dong, Gaochao Cho, William Chi-shing Ma, Patrick C. Brandi, Giovanni Tavolari, Simona Ujhazy, Peter Metro, Giulio Popper, Helmut H. Yin, Rong Qiu, Mantang Xu, Lin |
| Affiliation | Nanjing Med Univ, Affiliated Canc Hosp, Jiangsu Inst Canc Res,Dept Thorac Surg, Jiangsu Canc Hosp,Jiangsu Key Lab Mol & Translat, Nanjing 210009, Jiangsu, Peoples R China Yangzhou Univ, Affiliated Taixing Hosp, Taixing Peoples Hosp, Dept Cardiothorac Surg, Taixing 225400, Peoples R China Nanjing Med Univ, Collaborat Innovat Ctr Canc Personalized Med, Sch Publ Hlth, Dept Epidemiol & Biostat,Jiangsu Key Lab Canc Bio, Nanjing 210009, Jiangsu, Peoples R China Nanjing Med Univ, Jiangsu Canc Hosp, Jiangsu Inst Canc Res, Dept Sci Res,Affiliated Canc Hosp, Nanjing 210009, Jiangsu, Peoples R China Nanjing Univ, Sch Med, Jingling Hosp, Dept Comparat Med, Nanjing 210002, Jiangsu, Peoples R China Nanjing Med Univ, Dept Oncol, Affiliated Jiangning Hosp, Nanjing 211100, Jiangsu, Peoples R China Queen Elizabeth Hosp, Dept Clin Oncol, Hong Kong, Peoples R China Cleveland Clin, Taussig Canc Inst, Dept Solid Tumor Oncol, Aerodigest Oncol Translat Res THOR, Cleveland, OH 44106 USA S Orsola Malpighi Univ Hosp, Dept Expt Diagnost & Specialty Med, Bologna, Italy S Orsola Malpighi Univ Hosp, Ctr Appl Biomed Res, Bologna, Italy NCI, Translat Res Program, Div Canc Treatment & Diag, Bethesda, MD 20892 USA Azienda Osped Perugia, Santa Maria della Misericordia Hosp, Div Med Oncol, Via Dottori, I-106156 Perugia, Italy Med Univ Graz, Dept Pathol, Auenbruggerpl 25, A-8036 Graz, Austria Peking Univ, Peoples Hosp, Dept Thorac Surg, 11 South Xizhimen St, Beijing 100044, Peoples R China |
| Issue Date | 2019 |
| Publisher | MOLECULAR THERAPY-NUCLEIC ACIDS |
| Abstract | Emerging evidence demonstrates that long non-coding RNAs (lncRNAs) are deeply involved in the development of various cancers. This study identified that SBF2-AS1, an early-stagespecific lncRNA, is critical for the tumorigenesis of lung adenocarcinoma (LUAD). We first analyzed LUAD transcriptome data from The Cancer Genome Atlas and the GEO database by weighted gene co-expression network analysis (WGCNA). Five early LUAD-specific lncRNAs were filtered out, and only SBF2-AS1 was upregulated in LUAD. High expression of SBF2-AS1 indicates poor survival of LUAD, especially the early-stage LUAD, but not lung squamous cell carcinoma. SBF2-AS1 promotes LUAD cells proliferation in vitro, and RNA-sequencing data shows that many cell-cycle-related genes were downregulated after SBF2-AS1 knockdown. Mechanically, SBF2-AS1 could competitively bind with miR-338-3p and miR-362-3p to increase E2F1 expression. Finally, we show that the SBF2-AS1-miR-338-3p/362-3p-E2F1 axis could promote LUAD tumorigenesis in vitro and in vivo. Our study demonstrates that SBF2-AS1, an early-stage-specific lncRNA, promotes LUAD tumorigenesis by sponging miR-338-3p and miR-362-3p and increasing E2F1 expression. The SBF2-AS1-miR-338-3p/362-3p-E2F1 regulatory axis may serve as a prognostic marker and potential therapeutic target for LUAD. |
| URI | http://hdl.handle.net/20.500.11897/547543 |
| ISSN | 2162-2531 |
| DOI | 10.1016/j.omtn.2019.04.004 |
| Indexed | SCI(E) |
| Appears in Collections: | 人民医院 |
