| Title | Effects of perfluorooctanoic acid on stem Leydig cell functions in the rat |
| Authors | Lu, Hemin Zhang, Huishan Gao, Jie Li, Zhaohui Bao, Suhao Chen, Xianwu Wang, Yiyan Ge, Renshan Ye, Leping |
| Affiliation | Peking Univ, Dept Pediat, Hosp 1, 1 Xian Men St, Beijing 100034, Peoples R China Wenzhou Med Univ, Dept Pediat, Affiliated Hosp 2, Wenzhou 325027, Zhejiang, Peoples R China Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Zhejiang, Peoples R China Jinan Univ, Guangdong Prov Key Lab Bioengn Med, Dept Cell Biol, Guangzhou 510632, Guangdong, Peoples R China Wenzhou Med Univ, Dept Anesthesiol, Ctr Sci Res, Affiliated Hosp 2, Wenzhou 325027, Zhejiang, Peoples R China Wenzhou Med Univ, Yuying Childrens Hosp, 109 Western Xueyuan Rd, Wenzhou 325027, Zhejiang, Peoples R China |
| Keywords | Stem Leydig cells Leydig cell regeneration PFOA Steroidogenesis |
| Issue Date | 2019 |
| Publisher | ENVIRONMENTAL POLLUTION |
| Abstract | Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic (PFOS) are two perfluorinated chemical products widely existing in the environment. Evidence suggested that PFOA might relate to male reproductive dysfunction in rats and humans. PFOA exposure inhibited the function of Leydig cells. However, it is still unknown whether PFOA affects stem Leydig cells (SLCs). In the present study, we examined the effects of a short-term exposure to PFOA on Leydig cell regeneration and also explored the possible mechanism involved. Thirty-six adult Sprague-Dawley rats were randomly divided into three groups and intraperitoneally injected with a single dose of 75 mg/kg ethane dimethyl sulfonate (EDS) to eliminate all Leydig cells. From post-EDS day 7, the 3 group rats received 0, 25 or 50 mg/kg/day PFOA (n = 12 per group) for 9 consecutive days. Exposure to PFOA significantly decreased serum testosterone levels by day 21 and day 56 post-EDS treatment. Also, the expression levels of Leydig cell specific genes (Lhcgr, Scarb1, Star, Cyp11a1, Hsd3b1, Hsd11b1 and Cyp17a1) and their protein levels were all downregulated. PFOA exposure may also affect proliferation of SLCs or their progeny since the numbers of PCNA-positive Leydig cells were reduced by post-EDS day 21. These in vivo observations were also confirmed by in vitro studies where the effects of PFOA were tested by culture of seminiferous tubules. In summary, PFOA exposure inhibits the development of Leydig cells, possibly by affecting both the proliferation and differentiation of SLCs or their progeny. (C) 2019 Elsevier Ltd. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/547228 |
| ISSN | 0269-7491 |
| DOI | 10.1016/j.envpol.2019.03.120 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 第一医院 |
