| Title | Cobalt-Cross-Linked, Redox-Responsive Spy Network Protein Hydrogels |
| Authors | Kou, Songzi Yang, Xin Yang, Zhongguang Liu, Xiaotian Wegner, Seraphine V. Sun, Fei |
| Affiliation | Hong Kong Univ Sci & Technol, Shenzhen Peking Univ, Med Ctr, Biomed Res Inst, Shenzhen 518036, Peoples R China Hong Kong Univ Sci & Technol, Div Life Sci, State Key Lab Mol Neurosci, Hong Kong, Peoples R China Hong Kong Univ Sci & Technol, Ctr Syst Biol & Human Hlth, Sch Sci, Hong Kong, Peoples R China Hong Kong Univ Sci & Technol, Inst Adv Study, Hong Kong, Peoples R China Hong Kong Univ Sci & Technol, Dept Chem & Biol Engn, Hong Kong, Peoples R China Hong Kong Univ Sci & Technol, Ctr Syst Biol & Human Hlth, Hong Kong, Peoples R China Max Planck Inst Polymer Res, D-55128 Mainz, Germany |
| Issue Date | 2019 |
| Publisher | ACS MACRO LETTERS |
| Abstract | Although assembly of recombinant proteins by SpyTag/SpyCatcher chemistry has proven to be a versatile approach for creating bioactive hydrogels, the resulting Spy networks often exhibit weak mechanics due to the poor efficiency of interchain cross-linking. Here we leverage metal/ligand (i.e., cobalt/His6-tag) coordination interactions to modulate the bulk mechanics of the protein networks. The drastic difference between the Co2+ and Co3+ complexes in thermodynamic and kinetic properties enabled us to regulate the materials' properties and to immobilize and release recombinant proteins in a redox-dependent manner. The resulting hydrogels are capable of not only supporting cell growth and proliferation, but also influencing specific cell signaling via immobilized growth factors such as leukemia inhibitory factor (LIF). The integrated use of stimuli-responsive metal coordination and SpyTag/SpyCatcher chemistry opens up a new dimension for designing bioactive protein materials. |
| URI | http://hdl.handle.net/20.500.11897/546941 |
| DOI | 10.1021/acsmacrolett.9b00333 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 化学生物学与生物技术学院 |
