| Title | Molecular architecture of lineage allocation and tissue organization in early mouse embryo |
| Authors | Peng, Guangdun Suo, Shengbao Cui, Guizhong Yu, Fang Wang, Ran Chen, Jun Chen, Shirui Liu, Zhiwen Chen, Guoyu Qian, Yun Tam, Patrick P. L. Han, Jing-Dong J. Jing, Naihe |
| Affiliation | Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol Ce, Shanghai, Peoples R China Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, CAS Key Lab Regenerat Biol, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangzhou, Guangdong, Peoples R China Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, Guangzhou, Guangdong, Peoples R China Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China Chinese Acad Sci, CAS Key Lab Computat Biol,Shanghai Inst Nutr & Hl, CAS MPG Partner Inst Computat Biol,Collaborat Inn, Shanghai Inst Nutr & Hlth,Chinese Acad Sci Ctr Ex, Shanghai, Peoples R China Univ Sydney, Childrens Med Res Inst, Embryol Unit, Westmead, NSW, Australia Univ Sydney, Sch Med Sci, Fac Med & Hlth, Westmead, NSW, Australia Peking Univ, Acad Adv Interdisciplinary Studies, Peking Tsinghua Ctr Life Sci, CQB, Beijing, Peoples R China ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China Dana Farber Canc Inst, Dept Biostat & Computat Biol, Boston, MA 02115 USA Harvard TH Chan Sch Publ Hlth, Boston, MA USA |
| Issue Date | 2019 |
| Publisher | NATURE |
| Abstract | During post-implantation development of the mouse embryo, descendants of the inner cell mass in the early epiblast transit from the naive to primed pluripotent state(1). Concurrently, germ layers are formed and cell lineages are specified, leading to the establishment of the blueprint for embryogenesis. Fate-mapping and lineage-analysis studies have revealed that cells in different regions of the germ layers acquire location-specific cell fates during gastrulation(2-5). The regionalization of cell fates preceding the formation of the basic body plan-the mechanisms of which are instrumental for understanding embryonic programming and stem-cell-based translational study-is conserved in vertebrate embryos(6-8). However, a genome-wide molecular annotation of lineage segregation and tissue architecture of the post-implantation embryo has yet to be undertaken. Here we report a spatially resolved transcriptome of cell populations at defined positions in the germ layers during development from pre- to late-gastrulation stages. This spatiotemporal transcriptome provides high-resolution digitized in situ gene-expression profiles, reveals the molecular genealogy of tissue lineages and defines the continuum of pluripotency states in time and space. The transcriptome further identifies the networks of molecular determinants that drive lineage specification and tissue patterning, supports a role of Hippo-Yap signalling in germ-layer development and reveals the contribution of visceral endoderm to the endoderm in the early mouse embryo. |
| URI | http://hdl.handle.net/20.500.11897/545758 |
| ISSN | 0028-0836 |
| DOI | 10.1038/s41586-019-1469-8 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 前沿交叉学科研究院 |
