| Title | G-CSF-induced macrophage polarization and mobilization may prevent acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation |
| Authors | Wen, Qi Kong, Yuan Zhao, Hong-Yan Zhang, Yuan-Yuan Han, Ting-Ting Wang, Yu Xu, Lan-Ping Zhang, Xiao-Hui Huang, Xiao-Jun |
| Affiliation | Peking Univ, Inst Hematol, Peoples Hosp,Collaborat Innovat Ctr Hematol, Beijing Key Lab Hematopoiet Stem Cell Transplanta, Beijing, Peoples R China Southern Med Univ, Nanfang Hosp, Guangzhou, Guangdong, Peoples R China |
| Issue Date | 2019 |
| Publisher | BONE MARROW TRANSPLANTATION |
| Abstract | Macrophages (M Phi s) are an important immune cell population that are essential for tissue homeostasis and disease pathogenesis. M Phi s are now classified as either M1, which produce pro-inflammatory cytokines, or M2, which produce antiinflammatory cytokines. The impact of granulocyte colony-stimulating factor (G-CSF) on M Phi s in humans is unclear. Moreover, little is known about the association between M Phi subsets in allografts and the occurrence of acute graft-versushost disease (aGVHD) in patients who undergo allogeneic hematopoietic stem cell transplantation (allo-HSCT). In the current study, we found that the M1/M2 ratio was markedly decreased in both G-CSF-treated bone marrow (post-BM) and G-CSF-treated peripheral blood from healthy donors. Post-BM M Phi s exhibited reduced migration and increased phagocytosis. Moreover, post-BM M Phi s reduced the percentage of Th1 and Tc1 lineages and increased the percentage of Th2, Tc2, and Treg lineages. Patients who received BM grafts with a higher M1/M2 ratio exhibited a higher incidence of grade 2-4 aGVHD. In summary, our data indicate that G-CSF decreases the M1/M2 ratio in BM grafts from healthy donors, which may contribute to preventing the occurrence of grade 2-4 aGVHD in patients after allo-HSCT. |
| URI | http://hdl.handle.net/20.500.11897/545457 |
| ISSN | 0268-3369 |
| DOI | 10.1038/s41409-019-0449-9 |
| Indexed | SCI(E) |
| Appears in Collections: | 医学部待认领 |
