| Title | Integrin alpha 6 targeted positron emission tomography imaging of hepatocellular carcinoma in mouse models |
| Authors | Feng, Guo-Kai Ye, Jia-Cong Zhang, Wei-Guang Mei, Yan Zhou, Chao Xiao, Yi-Tai Li, Xin-Ling Fan, Wei Wang, Fan Zeng, Mu-Sheng |
| Affiliation | Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, Canc Ctr, State Key Lab Oncol South China, Guangzhou 510060, Guangdong, Peoples R China Peking Univ, Sch Basic Med Sci, Med Isotopes Res Ctr, Dept Radiat Med, Beijing 100191, Peoples R China |
| Keywords | Hepatocellular carcinoma Integrin alpha 6 Positron emission tomography Targeting peptide Phage display |
| Issue Date | 2019 |
| Publisher | JOURNAL OF CONTROLLED RELEASE |
| Abstract | Integrin alpha 6 emerges to be a diagnostic biomarker for hepatocellular carcinoma (HCC). Here, we translated our previously identified integrin alpha 6 targeted peptide RWY into a positron emission tomography (PET) tracer F-18-RWY for the detection of HCC lesions in following four HCC mouse models including subcutaneous, orthotopic, genetically engineered and chemical induced HCC mice. F-18-RWY produced high PET signals in liver tumor tissues that were reduced by blocking studies using nonradiolabeled RWY peptide. We compared the integrin alpha 6 targeted PET tracer F-18-RWY with the integrin alpha v beta 3-targeted PET tracer F-18-3PRGD2 and the clinical PET tracer F-18-FDG in chemical induced HCC mice. Among 12 HCC identified by enhanced magnetic resonance imaging (MRI) with hepatocellular specific gadoxetate disodium Gd-EOB-DTPA, the sensitivities of F-18-RWY, F-18-3PRGD2 and F-18-FDG were approximately 92%, 73% and 50% while the tumor-to-liver ratios were 4.36 +/- 1.41, 1.97 +/- 0.43 and 1.63 +/- 0.23 respectively. Additionally, PET imaging with the integrin alpha 6 targeted F-18-RWY enabled to visualize small HCC lesions with diameters approximately 0.2 cm that was hard to be distinguished from surround hepatic vascular by enhanced MRI with Gd-EOB-DTPA. These findings potentiate the use of integrin alpha 6 targeted PET tracer F-18-RWY for the detection of HCC. |
| URI | http://hdl.handle.net/20.500.11897/545020 |
| ISSN | 0168-3659 |
| DOI | 10.1016/j.jconrel.2019.08.003 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 基础医学院 |
