| Title | alpha-Helical Motif as Inhibitors of Toxic Amyloid-beta Oligomer Generation via Highly Specific Recognition of Amyloid Surface |
| Authors | Jiang, Yixiang Jiang, Xuehan Shi, Xiaodong Yang, Fadeng Cao, Yang Qin, Xuan Hou, Zhanfeng Xie, Mingsheng Liu, Na Fang, Qi Yin, Feng Han, Wei Li, Zigang |
| Affiliation | Peking Univ, Sch Chem Biol & Biotechnol, State Key Lab Chem Oncogen, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China Shenzhen Bay Lab, Shenzhen 518055, Peoples R China |
| Issue Date | 2019 |
| Publisher | ISCIENCE |
| Abstract | Amyloid fibril surfaces can convert soluble proteins into toxic oligomers and are attractive targets for intervention of protein aggregation diseases. Thus far, molecules identified with inhibitory activity are either large proteins or flat cyclic compounds lacking in specificity. The main design difficulty is flatness of amyloid surfaces and the lack of knowledge on binding interfaces. Here, we demonstrate, for the first time, a rational design of alpha-helical peptide inhibitors targeting the amyloid-beta 40 (A beta 40) fibril surfaces, based on our in silico finding that a helical fragment of A beta 40 interacts in a unique way with side-chain arrays on the fibril surface. We strengthen the fragment's binding capability through mutations and helicity enhancement with our Terminal Aspartic acid strategy. The resulting inhibitor shows micromolar affinity for the fibril surface, effectively impedes the surface-mediated oligomerization of A beta 40, and mitigates its cytotoxicity. This work opens up an avenue to designing aggregation modulators for amyloid diseases. |
| URI | http://hdl.handle.net/20.500.11897/543586 |
| ISSN | 2589-0042 |
| DOI | 10.1016/j.isci.2019.06.022 |
| Indexed | ESCI EI |
| Appears in Collections: | 化学生物学与生物技术学院 å å¦ä¸ å å å·¥ç¨ å¦é ¢ |
