| Title | Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells |
| Authors | He, Sicong Chen, Jiahao Jiang, Yunyun Wu, Yi Zhu, Lu Jin, Wan Zhao, Changlong Yu, Tao Wang, Tienan Wu, Shuting Lin, Xi Qu, Jianan Y. Wen, Zilong Zhang, Wenqing Xu, Jin |
| Affiliation | Hong Kong Univ Sci & Technol, Ctr Syst Biol & Human Hlth, Dept Elect & Comp Engn, Hong Kong, Hong Kong, Peoples R China. Southern Med Univ, Sch Basic Med Sci, Key Lab Zebrafish Modeling & Drug Screening Human, Dept Dev Biol, Guangzhou, Guangdong, Peoples R China. Hong Kong Univ Sci & Technol, Ctr Syst Biol & Human Hlth, Div Life Sci, Hong Kong, Hong Kong, Peoples R China. Hong Kong Univ Sci & Technol, Ctr Syst Biol & Human Hlth, State Key Lab Mol Neurosci, Hong Kong, Hong Kong, Peoples R China. Hong Kong Univ Sci & Technol, Shenzhen Peking Univ, Biomed Res Inst, Shenzhen Key Lab Neuronal Struct Biol,Med Ctr, Shenzhen, Peoples R China. South China Univ Technol, Sch Med, Div Cell Dev & Integrat Biol, Guangzhou, Guangdong, Peoples R China. Southern Med Univ, Sch Basic Med Sci, Key Lab Zebrafish Modeling & Drug Screening Human, Dept Dev Biol, Guangzhou, Guangdong, Peoples R China. Zhang, WQ Xu, J (reprint author), South China Univ Technol, Sch Med, Div Cell Dev & Integrat Biol, Guangzhou, Guangdong, Peoples R China. |
| Keywords | Langerhans cell,developmental biology,lineage tracing,regenerative medicine,stem cells,tissue resident macrophage,zebrafish TISSUE-RESIDENT MACROPHAGES STEM-CELLS C-MYB AORTIC ENDOTHELIUM STEADY-STATE IN-VIVO DEFINITIVE HEMATOPOIESIS GENE INDUCTION LYMPH-NODES YOLK-SAC |
| Issue Date | 2018 |
| Publisher | ELIFE |
| Citation | ELIFE. 2018, 7. |
| Abstract | The origin of Langerhans cells (LCs), which are skin epidermis-resident macrophages, remains unclear. Current lineage tracing of LCs largely relies on the promoter-Cre-LoxP system, which often gives rise to contradictory conclusions with different promoters. Thus, reinvestigation with an improved tracing method is necessary. Here, using a laser-mediated temporal-spatial resolved cell labeling method, we demonstrated that most adult LCs originated from the ventral wall of the dorsal aorta (VDA), an equivalent to the mouse aorta, gonads, and mesonephros (AGM), where both hematopoietic stem cells (HSCs) and non-HSC progenitors are generated. Further fine-fate mapping analysis revealed that the appearance of LCs in adult zebrafish was correlated with the development of HSCs, but not T cell progenitors. Finally, we showed that the appearance of tissue-resident macrophages in the brain, liver, heart, and gut of adult zebrafish was also correlated with HSCs. Thus, the results of our study challenged the EMP-origin theory for LCs. |
| URI | http://hdl.handle.net/20.500.11897/528401 |
| ISSN | 2050-084X |
| DOI | 10.7554/eLife.36131 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 化学生物学与生物技术学院 |
