| Title | AIE-based superwettable microchips for evaporation and aggregation induced fluorescence enhancement biosensing |
| Authors | Chen, Yanxia Min, Xuehong Zhang, Xiqi Zhang, Feilong Lu, Simeng Xu, Li-Ping Lou, Xiaoding Xia, Fan Zhang, Xueji Wang, Shutao |
| Affiliation | Univ Sci & Technol Beijing, Res Ctr Bioengn & Sensing Technol, Beijing Key Lab Bioengn & Sensing Technol, Sch Chem & Biol Engn, Beijing 100083, Peoples R China. Chinese Acad Sci, Tech Inst Phys & Chem, CAS Ctr Excellence Nanosci, CAS Key Lab Bioinspired Mat & Interfacial Sci, Beijing 100190, Peoples R China. China Univ Geosci, Fac Mat Sci & Chem, Wuhan 430074, Peoples R China. Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Wuhan 430074, Peoples R China. Peking Univ, Hlth Sci Ctr, Beijing 100191, Peoples R China. Univ Sci & Technol Beijing, Res Ctr Bioengn & Sensing Technol, Beijing Key Lab Bioengn & Sensing Technol, Sch Chem & Biol Engn, Beijing 100083, Peoples R China. Lou, XD (reprint author), China Univ Geosci, Fac Mat Sci & Chem, Wuhan 430074, Peoples R China. Lou, XD (reprint author), Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Wuhan 430074, Peoples R China. |
| Keywords | Superwettable microchip Aggregation-induced emission (AIE) Evaporation-induced enrichment Fluorescence enhancement Biosensing BLADDER-CANCER PATIENTS INDUCED EMISSION CONJUGATED POLYMERS BIOMEDICAL APPLICATIONS REAL-TIME DNA-PROBE PROTEIN SURFACE NANOPARTICLES MICRORNAS |
| Issue Date | 2018 |
| Publisher | BIOSENSORS & BIOELECTRONICS |
| Citation | BIOSENSORS & BIOELECTRONICS. 2018, 111, 124-130. |
| Abstract | Superwettable microchips with superhydrophilic microwells on superhydrophobic substrate have attracted increasing attention in fluorescence-based biological and medical diagnostics. However, traditional fluorophores often suffer from the aggregation-caused quenching (ACQ) problem at high concentration or in aggregated state. Here, we developed an AIE-based superwettable microchip by combining the evaporation-induced enrichment of superwettable microchips and the aggregation-induced emission of AlEgens together into one chip. Benefitting from the synergistic effect of the above two mechanisms, the AIE molecules (TPE-Z, a tetraphenylethene salt) were enriched from the diluted solution via evaporation and aggregated within the superhydrophilic microwell and then realized the fluorescence enhancement. Based on the dual enhancement effect of the ATE-based superwettable microchip, microRNA-141 (miR-141) can be detected with excellent reproducibility, sensitivity and specificity. A low detection limit of 1 pM can be achieved with higher signal-to-noise ratio than the traditional fluorescent probes. The proposed AIE-based superwettable microchip will provide a simple fluorescence enhancement biosensing platform for rapid, multiplexed and high-throughput analysis of specific targets in environmental monitoring, food safety, medical diagnosis and related research areas. |
| URI | http://hdl.handle.net/20.500.11897/523220 |
| ISSN | 0956-5663 |
| DOI | 10.1016/j.bios.2018.04.011 |
| Indexed | SCI(E) EI PubMed |
| Appears in Collections: | 医学部待认领 |
