| Title | Comparison of spatial chromosomal organization between bone marrow and peripheral blood in acute myeloid leukemia |
| Authors | Tian, Xueli Wang, Yanfang Liu, Yu Yin, Jun Chen, Dieyan Ke, Xiaoyan Ma, Wanyun |
| Affiliation | Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, 30 ShuangQing Rd, Beijing 100084, Peoples R China. Peking Univ, Hosp 3, Dept Hematol, 49 North Garden Rd, Beijing 100191, Peoples R China. Collaborat Innovat Ctr Quantum Matter, Beijing 100084, Peoples R China. Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, 30 ShuangQing Rd, Beijing 100084, Peoples R China. Ke, XY (reprint author), Peking Univ, Hosp 3, Dept Hematol, 49 North Garden Rd, Beijing 100191, Peoples R China. |
| Keywords | acute myeloid leukemia spatial organization bone marrow peripheral blood three-dimensional fluorescence in situ hybridization MINIMAL RESIDUAL DISEASE TIME QUANTITATIVE PCR IN-SITU HYBRIDIZATION ACUTE PROMYELOCYTIC LEUKEMIA AML 10 TRIAL GENE INTERPHASE SURVIVAL RELAPSE T(8/21) |
| Issue Date | 2018 |
| Publisher | ONCOLOGY LETTERS |
| Citation | ONCOLOGY LETTERS. 2018, 16(4), 4656-4662. |
| Abstract | Acute myeloid leukemia associated with t(8;21)(q22;q22)/runt related transcription factor (RUNX)1-RUNX1 translocation partner 1 has been reported to exhibit a favorable outcome. The quantitative polymerase chain reaction is a reliable method for assessing minimal residual disease persistence, and peripheral blood (PB) samples are as informative as bone marrow (BM) samples during follow-up monitoring. However, few studies have compared the spatial organization of leukemia-specific chromosomes between BM and PB. In the present study, paired BM and PB samples were extracted from 6 patients with acute myeloid leukaemia-M2 and compared using three-dimensional fluorescence in situ hybridization. Cells were classified into three types: Normal, proximal and malignant. Comparisons of proportions (% of all cells) of different cell types revealed no significant difference between BM and PB samples. The relative radial positions (RRPs; d/R) of chromosomes 8 and 21 were consistent for 2/3 of BM and PB samples. The RRPs of chromosomes in proximal pairs were more interior in nuclei compared with chromosomes in normal pairs for BM and PB samples. The consistency of the spatial organization of chromosomes between BM and PB suggests that PB may be an alternative to BM for research and clinical diagnosis. |
| URI | http://hdl.handle.net/20.500.11897/517133 |
| ISSN | 1792-1074 |
| DOI | 10.3892/ol.2018.9228 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 第三医院 |
