| Title | Efficient delivery of nucleic acid molecules into skin by combined use of microneedle roller and flexible interdigitated electroporation array |
| Authors | Huang, Dong Zhao, Deyao Wang, Xiaoxia Li, Chunhui Yang, Tongren Du, Lili Wei, Zewen Cheng, Qiang Cao, Huiqing Liang, Zicai Huang, Yuanyu Li, Zhihong |
| Affiliation | Peking Univ, Inst Mol Med, Inst Microelect, Natl Key Lab Sci & Technol Micro Nano Fabricat, Beijing 100871, Peoples R China. Beijing Inst Technol, Adv Res Inst Multidisciplinary Sci, Beijing 100081, Peoples R China. Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China. Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China. Peking Univ, Inst Mol Med, Inst Microelect, Natl Key Lab Sci & Technol Micro Nano Fabricat, Beijing 100871, Peoples R China. Huang, YY (reprint author), Beijing Inst Technol, Adv Res Inst Multidisciplinary Sci, Beijing 100081, Peoples R China. Huang, YY (reprint author), Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China. |
| Keywords | electroporation siRNA delivery gene therapy flexible interdigitated electroporation array microneedle roller TRANSDERMAL DRUG-DELIVERY IN-VIVO ELECTROPORATION GENE-THERAPY SIRNA DELIVERY DNA VACCINE INTRADERMAL ELECTROPORATION INSULIN DELIVERY B16 MELANOMA DESATURASE ELECTRODES |
| Issue Date | 2018 |
| Publisher | THERANOSTICS |
| Citation | THERANOSTICS. 2018, 8(9), 2361-2376. |
| Abstract | Rationale: Delivery of nucleic acid molecules into skin remains a main obstacle for various types of gene therapy or vaccine applications. Here we propose a novel electroporation approach via combined use of a microneedle roller and a flexible interdigitated electroporation array (FIEA) for efficient delivery of DNA and siRNA into mouse skin. Methods: Using micromachining technology, closely spaced gold electrodes were made on a pliable parylene substrate to form a patch-like electroporation array, which enabled close surface contact between the skin and electrodes. Pre-penetration of the skin with a microneedle roller resulted in the formation of microchannels in the skin, which played a role as liquid electrodes in the skin and provided a uniform and deep electric field in the tissue when pulse stimulation was applied by FIEA. Results: Using this proposed method, gene (RFP) expression and siRNA transfection were successfully achieved in normal mice skin. Anti-SCD1 siRNA electroporated via this method mediated significant gene silencing in the skin. Moreover, electroporation assisted by the microneedle roller showed significant advantages over treatment with FIEA alone. This allowed nucleic acid transportation at low voltage, with ideal safety outcomes. Principal conclusions: Hence, the proposed electroporation approach in this study constitutes a novel way for delivering siRNA and DNA, and even other nucleic acid molecules, to mouse skin in vivo, potentially supporting clinical application in the treatment of skin diseases or intradermal/subcutaneous vaccination. |
| URI | http://hdl.handle.net/20.500.11897/512868 |
| ISSN | 1838-7640 |
| DOI | 10.7150/thno.23438 |
| Indexed | SCI(E) PubMed Medline |
| Appears in Collections: | 分子医学研究所 |
