| Title | Engineering peptidoglycan degradation related genes of Bacillus subtilis for better fermentation processes |
| Authors | Zhao, Liang Ye, Jianwen Fu, Jing Chen, Guo-Qiang |
| Affiliation | Tsinghua Univ, Sch Life Sci, Peking Tsinghua Ctr Life Sci, Beijing 100084, Peoples R China. Tsinghua Univ, Ctr Synthet & Syst Biol, Beijing 100084, Peoples R China. Tsinghua Univ, MOE Key Lab Ind Biocatalysis, Beijing 100081, Peoples R China. Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100081, Peoples R China. Tianjin Univ, Sch Chem Engn & Technol, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Key Lab Syst Bioengn,Minist Educ,SynBio Res Platf, Tianjin 300072, Peoples R China. |
| Keywords | Genome edition Peptidoglycan hydrolase Bacillus subtilis Morphology engineering alpha-Amylase POLYACRYLAMIDE-GEL ELECTROPHORESIS CELL-WALL HYDROLASES VEGETATIVE GROWTH BACTERIAL THREAD AUTOLYSINS GLUCOSAMINIDASE LYTF SEPARATION DEFICIENT RESISTANT |
| Issue Date | 2018 |
| Publisher | BIORESOURCE TECHNOLOGY |
| Citation | BIORESOURCE TECHNOLOGY. 2018, 248, 238-247. |
| Abstract | In this study, Bacillus subtilis 168 Dupp was engineered to change the bacterial shapes. Namely, some peptidoglycan hydrolase related genes were inactivated individually or in different combinations, including sigD, lytE, lytF, lytC, lytD and lytG. Inactivations of these genes resulted in various intensities of blockages on cell division, leading to elongation of bacterial cells. The resulted fiber phenotypes showed different lengths ranging from tens of microns to several millimeters. Mutants with multiple gene inactivations such as Delta sigD Delta lytE Delta lytD showed more easily precipitated phenomenon, obviously increased growth rate, more sensitive to antibiotics and improved alpha-amylase production compared with that of B. subtilis 168 Dupp. Mutants Delta sigD Delta lytE Delta lytD and Delta sigD Delta lytE Delta lytC Delta lytD also showed an increased tolerance to high osmotic pressure of sodium chloride, allowing unsterile fermentation, all of which contributes to reduced processing cost. (C) 2017 Elsevier Ltd. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/500235 |
| ISSN | 0960-8524 |
| DOI | 10.1016/j.biortech.2017.05.134 |
| Indexed | SCI(E) EI PubMed Medline |
| Appears in Collections: | 前沿交叉学科研究院 |
