Title | Development and Validation of a New Serum Standard for the Measurement of Anti-HEV Antibodies in Animals |
Authors | Zhang, Yulin Wang, Lin Zeng, Hang Gong, Wanyun Wang, Ling Zhuang, Hui |
Affiliation | Peking Univ, Hlth Sci Ctr, Dept Microbiol, 38 Xueyuan Rd, Beijing 100191, Peoples R China. Peking Univ, Hlth Sci Ctr, Ctr Infect Dis, Sch Basic Med Sci, 38 Xueyuan Rd, Beijing 100191, Peoples R China. Peking Univ, Hlth Sci Ctr, Dept Microbiol, 38 Xueyuan Rd, Beijing 100191, Peoples R China. Wang, L Zhuang, H (reprint author), Peking Univ, Hlth Sci Ctr, Ctr Infect Dis, Sch Basic Med Sci, 38 Xueyuan Rd, Beijing 100191, Peoples R China. |
Keywords | HEV immune response diagnostic test animals ELISA quantitation HEPATITIS-E-VIRUS E VACCINE ZOONOTIC TRANSMISSION DOMESTIC-ANIMALS INFECTION RABBITS CHINA PREVALENCE |
Issue Date | 2017 |
Publisher | JOURNAL OF MEDICAL VIROLOGY |
Citation | JOURNAL OF MEDICAL VIROLOGY.2017,89(3),497-501. |
Abstract | Although hepatitis E virus (HEV) infection is a zoonosis, there is, currently, no standardized assay for quantitatively measuring anti-HEV antibody levels in HEV animal reservoirs. In this study, anti-HEV antibody positive serum from a rabbit (RS26) was calibrated by dose-response parallel line assay using the World Health Organization (WHO) reference standard for anti-HEV antibodies. After evaluating the stability of the RS26 serum, a quantification assay of anti-HEV antibodies using RS26 as a standard was developed and evaluated for both reproducibility and suitability to field studies. The anti-HEV antibody level of RS26 was calculated to be 39.54 IU/ml. A series of standard working serum for anti-HEV antibodies consisted of five serum dilutions (3.950 IU/ml, 1.975 IU/ml, 0.986 IU/ml, 0.494 IU/ml, and 0.247 IU/ml). The quantitative assay using RS26 showed good range and reproducibility, effectively measuring the dynamics of anti-HEV antibody concentrations in pigs and rabbits. In conclusion, we have developed a a stable and reproducible serum standard for the quantitation of anti-HEV antibodies. (C) 2016 Wiley Periodicals, Inc. |
URI | http://hdl.handle.net/20.500.11897/493329 |
ISSN | 0146-6615 |
DOI | 10.1002/jmv.24651 |
Indexed | SCI(E) PubMed |
Appears in Collections: | 医学部待认领 |