| Title | Macrophage migration inhibitory factor contributes to anti-neutrophil cytoplasmic antibody-induced neutrophils activation |
| Authors | Hao, Jian Lv, Tie-Gang Wang, Chen Xu, Li-Ping Zhao, Jian-Rong |
| Affiliation | Inner Mongolia Med Coll, Affiliated Hosp, Dept Med, Div Renal, Hohhot 010050, Inner Mongolia, Peoples R China. Peking Univ, Key Lab Renal Dis, Minist Hlth China, Renal Div,Dept Med,Hosp 1, Beijing 100034, Peoples R China. Inner Mongolia Med Coll, Dept Pharmacol, Hohhot 010010, Inner Mongolia, Peoples R China. |
| Keywords | Macrophage migration inhibitory factor ANCA Neutrophils ANCA-ASSOCIATED VASCULITIS SYSTEMIC-LUPUS-ERYTHEMATOSUS RHEUMATOID-ARTHRITIS ANTIMYELOPEROXIDASE ANTIBODIES CRESCENTIC GLOMERULONEPHRITIS SYNOVIAL FIBROBLASTS CHEMOKINE RECEPTORS CELL RECRUITMENT FACTOR MIF TNF-ALPHA |
| Issue Date | 2016 |
| Publisher | HUMAN IMMUNOLOGY |
| Citation | HUMAN IMMUNOLOGY.2016,77(12),1209-1214. |
| Abstract | Background: Macrophage migration inhibitory factor (MIF) is an inflammatory mediator released by macrophages that is central to the innate immune system, with an upstream role in the inflammatory cascade. MIF is one of the most important pathogenic factors in the development of the autoimmune diseases. In the current study, we investigated the role of MIF in anti-neutrophil cytoplasmic antibody (ANCA)-induced neutrophil activation. Methods: Plasma levels of MIF from 31 patients with active ANCA-associated vasculitis (AAV) were analyzed by ELISA. The various effects of MIF in ANCA-induced neutrophil respiratory burst and degranulation were measured. Results: Plasma levels of circulating MIF were significantly higher in AAV patients with active disease compared with those in remission and healthy controls. Compared with MIF-primed neutrophils, the MFI value increased significantly in MIF-primed neutrophils further activated with MPO-ANCA-positive IgG or PR3-ANCA-positive IgG (270.8 +/- 9.7 vs. 421.5 +/- 9.7, P < 0.001; 270.8 +/- 9.7 vs. 414.1 +/- 15.6, P < 0.001, respectively). Compared with MIF-primed neutrophils, the lactoferrin concentration increased significantly in the supernatant of MIF-primed neutrophils further activated by MPO-ANCA-positive IgG (567.8 +/- 61.2 ng/ml vs. 1677.0 +/- 42.5 ng/ml, P < 0.001) or PR3-ANCA-positive IgG (567.8 +/- 61.2 ng/ml vs. 1546.0 +/- 116.2 ng/ml, P < 0.001), respectively. Interleukin-8 (IL-8), IL-6 and IL-23 were involved in ANCA-induced activation of MIF-primed neutrophils. Conclusions: MIF primes neutrophils by increasing ANCA antigen translocation. The primed neutrophils can be further induced by ANCA, resulting in respiratory burst and degranulation. (C) 2016 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/493150 |
| ISSN | 0198-8859 |
| DOI | 10.1016/j.humimm.2016.08.006 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 第一医院 |
