| Title | Neuroprotective Effects of a Standardized Flavonoid Extract from Safflower against a Rotenone-Induced Rat Model of Parkinson's Disease |
| Authors | Ablat, Nuramatjan Lv, Deyong Ren, Rutong Xiaokaiti, Yilixiati Ma, Xiang Zhao, Xin Sun, Yi Lei, Hui Xu, Jiamin Ma, Yingcong Qi, Xianrong Ye, Min Xu, Feng Han, Hongbin Pu, Xiaoping |
| Affiliation | Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China. Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China. Peking Univ, Dept Radiol, Hosp 3, Beijing 100191, Peoples R China. Dongying Peoples Hosp Shandong, Dept Radiol, Dongying 257091, Peoples R China. Peking Univ, Sch Basic Med Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China. Peking Univ, Hosp 3, Beijing Key Lab MRI Device & Tech, Beijing 100191, Peoples R China. Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China. Pu, XP (reprint author), Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China. Han, HB (reprint author), Peking Univ, Dept Radiol, Hosp 3, Beijing 100191, Peoples R China. Han, HB (reprint author), Peking Univ, Hosp 3, Beijing Key Lab MRI Device & Tech, Beijing 100191, Peoples R China. |
| Keywords | safflower flavonoid extract Parkinson's disease DA MRI PERFORMANCE LIQUID-CHROMATOGRAPHY CARTHAMUS-TINCTORIUS L. AMBIENT MASS-SPECTROMETRY FLOW-ASSISTED IONIZATION HEAT-SHOCK PROTEINS EXTRACELLULAR-SPACE DOPAMINERGIC-NEURONS IN-VIVO DIFFUSION BRAIN |
| Issue Date | 2016 |
| Publisher | MOLECULES |
| Citation | MOLECULES.2016,21(9). |
| Abstract | Parkinson's disease (PD) is a major age-related neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra par compacta (SNpc). Rotenone is a neurotoxin that is routinely used to model PD to aid in understanding the mechanisms of neuronal death. Safflower (Carthamus tinctorius. L.) has long been used to treat cerebrovascular diseases in China. This plant contains flavonoids, which have been reported to be effective in models of neurodegenerative disease. We previously reported that kaempferol derivatives from safflower could bind DJ-1, a protein associated with PD, and that a flavonoid extract from safflower exhibited neuroprotective effects in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of PD. In this study, a standardized safflower flavonoid extract (SAFE) was isolated from safflower and found to primarily contain flavonoids. The aim of the current study was to confirm the neuroprotective effects of SAFE in rotenone-induced Parkinson rats. The results showed that SAFE treatment increased body weight and improved rearing behavior and grip strength. SAFE (35 or 70 mg/kg/day) treatment reversed the decreased protein expression of tyrosine hydroxylase, dopamine transporter and DJ-1 and increased the levels of dopamine and its metabolite. In contrast, acetylcholine levels were decreased. SAFE treatment also led to partial inhibition of PD-associated changes in extracellular space diffusion parameters. These changes were detected using a magnetic resonance imaging (MRI) tracer-based method, which provides novel information regarding neuronal loss and astrocyte activation. Thus, our results indicate that SAFE represents a potential therapeutic herbal treatment for PD. |
| URI | http://hdl.handle.net/20.500.11897/493074 |
| ISSN | 1420-3049 |
| DOI | 10.3390/molecules21091107 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 药学院 第三医院 基础医学院 天然药物与仿生药物国家重点实验室 |
