| Title | 8-C-(E-phenylethenyl)quercetin from onion/beef soup induces autophagic cell death in colon cancer cells through ERK activation |
| Authors | Zhao, Yueliang Fan, Daming Zheng, Zong-Ping Li, Edmund T. S. Chen, Feng Cheng, Ka-Wing Wang, Mingfu |
| Affiliation | Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China. Peking Univ, Inst Food & Bioresource Engn, Coll Engn, Beijing, Peoples R China. |
| Keywords | Autophagy Colon cancer ERK activation 8-C-(E-phenylethenyl)quercetin Onion beef soup HETEROCYCLIC AMINE FORMATION MAILLARD REACTION BEEF PATTIES SIGNALING PATHWAY PHENYLACETALDEHYDE INHIBITION PREVENTION THERAPY DISEASE GROWTH |
| Issue Date | 2017 |
| Publisher | MOLECULAR NUTRITION & FOOD RESEARCH |
| Citation | MOLECULAR NUTRITION & FOOD RESEARCH.2017,61(2). |
| Abstract | ScopeQuercetin, a flavonoid, widely distributed in edible fruits and vegetables, was reported to effectively inhibit 2-amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP) formation in a food model (roast beef patties) with itself being converted into a novel compound 8-C-(E-phenylethenyl)quercetin (8-CEPQ). Here we investigated whether 8-CEPQ could be formed in a real food system, and tested its anticancer activity in human colon cancer cell lines. Methods and resultsLC-MS was applied for the determination of 8-CEPQ formation in onion/beef soup. Anticancer activity of 8-CEPQ was evaluated by using cell viability assay and flow cytometry. Results showed that 8-CEPQ suppressed proliferationand caused G(2) phase arrest in colon cancer cells. Based on immunofluorescent staining assay, western blot assay, and RNA knockdown data, we found that 8-CEPQ did not cause apoptotic cell death. Instead, it induced autophagic cell death. Moreover, treatment with 8-CEPQ induced phosphorylation of extracellular signal-regulated kinase (ERK). Inhibition of ERK phosphorylation by the mitogen-activated protein kinasekinase (MEK)/ERK inhibitor U0126 attenuated 8-CEPQ-induced autophagy and reversed 8-CEPQ-mediated cell growth inhibition. ConclusionOur results demonstrate that 8-CEPQ, a novel quercetin derivative, could be formed in onion/beef soup. 8-CEPQ inhibited colon cancer cell growth by inducing autophagic cell death through ERK activation. |
| URI | http://hdl.handle.net/20.500.11897/492709 |
| ISSN | 1613-4125 |
| DOI | 10.1002/mnfr.201600437 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 工学院 |
