| Title | Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Contribute to Chondrogenesis in Coculture with Chondrocytes |
| Authors | Li, Xingfu Duan, Li Liang, Yujie Zhu, Weimin Xiong, Jianyi Wang, Daping |
| Affiliation | Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen Key Lab Tissue Engn, Shenzhen 518035, Guangdong, Peoples R China. Shenzhen Univ, Dept Orthoped, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen 518035, Guangdong, Peoples R China. Chinese Univ Hong Kong, Dept Chem, Shatin, Hong Kong, Peoples R China. Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Shenzhen 518055, Guangdong, Peoples R China. Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen Key Lab Tissue Engn, Shenzhen 518035, Guangdong, Peoples R China. Xiong, JY Wang, DP (reprint author), Shenzhen Univ, Dept Orthoped, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen 518035, Guangdong, Peoples R China. |
| Keywords | HUMAN ARTICULAR CHONDROCYTES BONE-MARROW IN-VITRO DIFFERENTIATION STATE MATRIX COMPONENTS HYPERTROPHY EXPRESSION CARTILAGE PHENOTYPE CULTURE |
| Issue Date | 2016 |
| Publisher | BIOMED RESEARCH INTERNATIONAL |
| Citation | BIOMED RESEARCH INTERNATIONAL.2016. |
| Abstract | Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have been shown as the most potential stem cell source for articular cartilage repair. In this study, we aimed to develop a method for long-term coculture of human articular chondrocytes (hACs) and hUCB-MSCs at low density in vitro to determine if the low density of hACs could enhance the hUCB-MSC chondrogenic differentiation as well as to determine the optimal ratio of the two cell types. Also, we compared the difference between direct coculture and indirect coculture at low density. Monolayer cultures of hUCB-MSCs and hACs were investigated at different ratios, at direct cell-cell contact groups for 21 days. Compared to direct coculture, hUCB-MSCs and hACs indirect contact culture significantly increased type II collagen (COL2) and decreased type I collagen (COL1) protein expression levels. SRY-box 9 (SOX9) mRNA levels and protein expression were highest in indirect coculture. Overall, these results indicate that low density direct coculture induces fibrocartilage. However, indirect coculture in conditioned chondrocyte cell culture medium can increase expression of chondrogenic markers and induce hUCB-MSCs differentiation into mature chondrocytes. This work demonstrates that it is possible to promote chondrogenesis of hUCB-MSCs in combination with hACs, further supporting the concept of novel coculture strategies for tissue engineering. |
| URI | http://hdl.handle.net/20.500.11897/492509 |
| ISSN | 2314-6133 |
| DOI | 10.1155/2016/3827057 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 化学生物学与生物技术学院 |
