Title | Arabidopsis DET1 Represses Photomorphogenesis in Part by Negatively Regulating DELLA Protein Abundance in Darkness |
Authors | Li, Kunlun Gao, Zhaoxu He, Hang Terzaghi, William Fan, Liu-Min Deng, Xing Wang Chen, Haodong |
Affiliation | Peking Univ, Peking Tsinghua Ctr Life Sci, Coll Life Sci,State Key Lab Prot & Plant Gene Res, Peking Yale Joint Ctr Plant Mol Genet & Agrobiote, Beijing 100871, Peoples R China. Wilkes Univ, Dept Biol, Wilkes Barre, PA 18766 USA. Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA. |
Keywords | DET1 DELLA gibberellic acid (GA) photomorphogenesis Arabidopsis LIGHT CONTROL GIBBERELLIN RESPONSES TRANSCRIPTION FACTOR SEEDLING DEVELOPMENT RECEPTOR GID1 GENE EXPRESSION PATHWAY DESTABILIZATION RECOGNITION |
Issue Date | 2015 |
Publisher | MOLECULAR PLANT |
Citation | MOLECULAR PLANT.2015,8,(4),622-630. |
Abstract | Arabidopsis De-etiolated 1 (DET1) is one of the key repressors that maintain the etiolated state of seedlings in darkness. The plant hormone gibberellic acid (GA) also participates in this process, and plants deficient in GA synthesis or signaling show a partially de-etiolated phenotype in darkness. However, how DET1 and the GA pathwaywork in concert in repressing photomorphogenesis remains largely unknown. In this study, we found that the abundance of DELLA proteins in det1-1 was increased in comparison with that in the wild-type plants. Mutation in DET1 changed the sensitivity of hypocotyl elongation of mutant seedlings to GA and paclobutrazol (PAC), an inhibitor of GA synthesis. However, we did not find obvious differences between det1-1 and wild-type plants with regard to the bioactive GA content or the GA signaling upstream of DELLAs. Genetic data showed that removal of several DELLA proteins suppressed the det1-1 mutant phenotype more obviously than GA treatment, indicating that DET1 can regulate DELLA proteins via some other mechanisms. In addition, a large-scale transcriptomic analysis revealed that DET1 and DELLAs play antagonistic roles in regulating expression of photosynthetic and cell elongation-related genes in etiolated seedlings. Taken together, our results show that DET1 represses photomorphogenesis in darkness in part by reducing the abundance of DELLA proteins. |
URI | http://hdl.handle.net/20.500.11897/492297 |
ISSN | 1674-2052 |
DOI | 10.1016/j.molp.2014.12.017 |
Indexed | SCI(E) 中国科技核心期刊(ISTIC) 中国科学引文数据库(CSCD) |
Appears in Collections: | 生命科学学院 |