| Title | Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to induce liver tumour formation in mice via AKT2/mTORC1 cascade |
| Authors | Wang, Chunmei Che, Li Hu, Junjie Zhang, Shanshan Jiang, Lijie Latte, Gavinella Demartis, Maria I. Tao, Junyan Gui, Bing Pilo, Maria G. Ribback, Silvia Dombrowski, Frank Evert, Matthias Calvisi, Diego F. Chen, Xin |
| Affiliation | Univ Calif San Francisco, Dept Bioengineering & Therapeut Sci & Liver Ctr, San Francisco, CA USA. Peking Univ Canc Hosp & Inst, Div Oncol Key Lab Carcinogenesis & Translat Res, Minist Educ, Beijing, Peoples R China. Hubei Univ Chinese Med, Sch Pharm, Wuhan, Hunan, Peoples R China. Second Mil Med Univ, Dept Pathol Eastern Hepatobiliary Surg Hosp, Shanghai, Peoples R China. Univ Sassari, Dept Clin & Expt Med, Sassari, Italy. Ernst Moritz Arndt Univ Greifswald, Inst Pathol, Greifswald, Germany. Boston Childrens Hosp, Harvard Med Sch, Div Endocrinol, Boston, MA USA. Univ Regensburg, Inst Pathol, Regensburg, Germany. Dept Clin & Expt Med, Correspondence, Diego F Calvisi, I-07100 Sassari, 228306, Italy. |
| Keywords | AKT/mTOR c-Met liver cancer NRasV12 PIK3CA mutants HEPATOCELLULAR-CARCINOMA HUMAN CANCER COMPLEX 1 PTEN-LOSS PATHWAY TARGET MOUSE AKT2 HEPATOCARCINOGENESIS OPPORTUNITIES |
| Issue Date | 2016 |
| Publisher | LIVER INTERNATIONAL |
| Citation | LIVER INTERNATIONAL.2016,36(8),1176-1186. |
| Abstract | Background & AimsActivating mutations of PIK3CA occur in various tumour types, including human hepatocellular carcinoma. The mechanisms whereby PIK3CA contributes to hepatocarcinogenesis remain poorly understood. MethodsPIK3CA mutants H1047R or E545K were hydrodynamically transfected, either alone or in combination with NRasV12 or c-Met genes, in the mouse liver. ResultsOverexpression of H1047R or E545K alone was able to induce AKT/mTOR signalling in the mouse liver, leading to hepatic steatosis. However, none of the mice developed liver tumours over long term. In contrast, H1047R or E545K cooperated with NRasV12 or c-Met to rapidly induce liver tumour formation in mice. At the molecular level, all the tumour nodules displayed activation of AKT/mTOR and Ras/MAPK cascades. Ablation of AKT2 significantly inhibited hepatic steatosis induced by H1047R or E545K and carcinogenesis induced by H1047R/c-Met or E545K/c-Met. Furthermore, tumourigenesis induced by H1047R/c-Met was abolished in conditional Raptor knockout mice. ConclusionsBoth H1047R and E545K are able to activate the AKT/mTOR pathway. An intact AKT2/mTOR complex 1 cascade is required for tumourigenesis induced by H1047R/c-Met or E545K/c-Met in the liver. |
| URI | http://hdl.handle.net/20.500.11897/491808 |
| ISSN | 1478-3223 |
| DOI | 10.1111/liv.13055 |
| Indexed | SCI(E) |
| Appears in Collections: | 北京肿瘤医院 |
