| Title | Fabrication and In Vitro Study of Tissue-Engineered Cartilage Scaffold Derived from Wharton's Jelly Extracellular Matrix |
| Authors | Xiao, Tongguang Guo, Weimin Chen, Mingxue Hao, Chunxiang Gao, Shuang Huang, Jingxiang Yuan, Zhiguo Zhang, Yu Wang, Mingjie Li, Penghao Peng, Jiang Wang, Aiyuan Wang, Yu Sui, Xiang Zhang, Li Xu, Wenjing Lu, Shibi Yin, Heyong Yang, Jianhua Liu, Shuyun Guo, Quanyi |
| Affiliation | PLA, Key Lab Musculoskeletal Trauma & War Injuries, Beijing Key Lab Regenerat Med Orthopaed, Inst Orthopaed,Chinese PLA Gen Hosp, 28 Fuxing Rd, Beijing 100853, Peoples R China. Jiamusi Univ, Dept Orthopaed 1, Affiliated Hosp 1, 348 Dexiang Rd, Jiamusi 154003, Peoples R China. Chinese Peoples Liberat Army Gen Hosp, Inst Anesthesiol, 28 Fuxing Rd, Beijing 100853, Peoples R China. Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomat & Tissue Engn, 5 Yiheyuan Rd, Beijing 100871, Peoples R China. PLA, Key Lab Musculoskeletal Trauma & War Injuries, Beijing Key Lab Regenerat Med Orthopaed, Inst Orthopaed,Chinese PLA Gen Hosp, 28 Fuxing Rd, Beijing 100853, Peoples R China. Yang, JH (reprint author), Jiamusi Univ, Dept Orthopaed 1, Affiliated Hosp 1, 348 Dexiang Rd, Jiamusi 154003, Peoples R China. |
| Keywords | CHONDROCYTE IMPLANTATION COLLAGEN GENE REGENERATION REPAIR BONE CELLS DECELLULARIZATION MICROFRACTURE DEFECTS JOINT |
| Issue Date | 2017 |
| Publisher | BIOMED RESEARCH INTERNATIONAL |
| Citation | BIOMED RESEARCH INTERNATIONAL. 2017. |
| Abstract | The scaffold is a key element in cartilage tissue engineering. The components of Wharton's jelly are similar to those of articular cartilage and it also contains some chondrogenic growth factors, such as insulin-like growth factor I and transforming growth factor-beta. We fabricated a tissue-engineered cartilage scaffold derived from Wharton's jelly extracellular matrix (WJECM) and compared it with a scaffold derived from articular cartilage ECM(ACECM) using freeze-drying. The results demonstrated that both WJECM and ACECM scaffolds possessed favorable pore sizes and porosities; moreover, they showed good water uptake ratios and compressive moduli. Histological staining confirmed that the WJECM and ACECM scaffolds contained similar ECM. Moreover, both scaffolds showed good cellular adherence, bioactivity, and biocompatibility. MTT and DNA content assessments confirmed that the ACECM scaffold tended to be more beneficial for improving cell proliferation than the WJECM scaffold. However, RTq-PCR results demonstrated that the WJECM scaffold was more favorable to enhance cellular chondrogenesis than the ACECM scaffold, showing more collagen II and aggrecan mRNA expression. These results were confirmed indirectly by glycosaminoglycan and collagen content assessments and partially confirmed by histology and immunofluorescent staining. In conclusion, these results suggest that a WJECM scaffold may be favorable for future cartilage tissue engineering. |
| URI | http://hdl.handle.net/20.500.11897/484871 |
| ISSN | 2314-6133 |
| DOI | 10.1155/2017/5839071 |
| Indexed | SCI(E) |
| Appears in Collections: | 前沿交叉学科研究院 |
