| Title | Interleukin-17A promotes tongue squamous cell carcinoma metastasis through activating miR-23b/versican pathway |
| Authors | Wei, Tai Cong, Xin Wang, Xiang-Ting Xu, Xiao-Jian Min, Sai-Nan Ye, Peng Peng, Xin Wu, Li-Ling Yu, Guang-Yan |
| Affiliation | Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Natl Engn Lab Digital & Mat Technol Stomatol, Beijing, Peoples R China. Beijing Key Lab Digital Stomatol, Beijing, Peoples R China. Peking Univ, Sch Basic Med Sci, Key Lab Mol Cardiovasc Sci, Dept Physiol & Pathophysiol,Minist Educ, Beijing, Peoples R China. Beijing Key Lab Cardiovasc Receptors Res, Beijing, Peoples R China. Univ Sci & Technol China, Sch Life Sci, Dept Cell & Dev Biol, Hefei, Peoples R China. Peking Univ, Hosp 3, Dept Ophthalmol, Beijing, Peoples R China. Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Natl Engn Lab Digital & Mat Technol Stomatol, Beijing, Peoples R China. Yu, GY (reprint author), Beijing Key Lab Digital Stomatol, Beijing, Peoples R China. Wu, LL (reprint author), Peking Univ, Sch Basic Med Sci, Key Lab Mol Cardiovasc Sci, Dept Physiol & Pathophysiol,Minist Educ, Beijing, Peoples R China. Wu, LL (reprint author), Beijing Key Lab Cardiovasc Receptors Res, Beijing, Peoples R China. |
| Keywords | interleukin-17A miR-23b versican tongue squamous cell carcinoma metastasis STROMAL VERSICAN EXPRESSION CANCER CELLS KAPPA-B CLINICOPATHOLOGICAL FACTORS PROTEOGLYCAN SYNTHESIS CYTOKINE EXPRESSION ANTITUMOR-ACTIVITY PROSTATE-CANCER BLADDER-CANCER OVARIAN-CANCER |
| Issue Date | 2017 |
| Publisher | ONCOTARGET |
| Citation | ONCOTARGET.2017,8(4),6663-6680. |
| Abstract | Interleukin-17A (IL-17A), a proinflammatory cytokine mainly produced by T helper 17 cells, exerts protumor or antitumor effects in different cancer entities. However, the exact role of IL-17A in carcinogenesis and progression of tongue squamous cell carcinoma (TSCC) remains unclear. Here, we found that the levels of IL-17A in serum and tumor samples were significantly increased in TSCC patients and positively correlated with tumor metastasis and clinical stage. Besides, IL-17A enhanced cell migration and invasion in SCC15, a TSCC cell line. Furthermore, IL-17A inversely correlated with miR-23b expression in TSCC specimens. In vitro, NF-kappa B inhibited miR-23b transcription by directly binding to its promoter region. IL-17A downregulated miR-23b expression via activating NF-kappa B signaling pathway characterized by increasing p65 expression in the nuclear and elevating the levels of p-IKKa and p-I kappa Ba. Overexpression of miR-23b inhibited, whereas knockdown of miR-23b promoted migration and invasion abilities of SCC15 cells. Moreover, extracellular matrix protein versican was proved to be the direct target of miR-23b through luciferase assay. IL-17A increased versican levels in vitro and knockdown of versican by siRNA inhibited SCC15 cell migration and invasion. Taken together, these results reveal a novel mechanism that IL-17A in TSCC microenvironment promotes the migration and invasion of TSCC cells through targeting miR-23b/versican pathway. |
| URI | http://hdl.handle.net/20.500.11897/475609 |
| ISSN | 1949-2553 |
| DOI | 10.18632/oncotarget.14255 |
| Indexed | SCI(E) |
| Appears in Collections: | 口腔医院 基础医学院 第三医院 |
