| Title | C9ORF135 encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells |
| Authors | Zhou, Shixin Liu, Yinan Ma, Yumin Zhang, Xiaoyan Li, Yang Wen, Jinhua |
| Affiliation | Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Sch Basic Med Sci, Beijing 100191, Peoples R China. Peking Univ, Dept Cell Biol, Beijing 100191, Peoples R China. Peking Univ, Peking Univ Diabet Ctr, Peoples Hosp, Beijing 100044, Peoples R China. Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Sch Basic Med Sci, Beijing 100191, Peoples R China. Wen, JH (reprint author), Peking Univ, Dept Cell Biol, Beijing 100191, Peoples R China. |
| Keywords | HUMAN FIBROBLASTS IN-VITRO DIFFERENTIATION NEURONS GENERATION DERIVATION MARKERS REVEAL |
| Issue Date | 2017 |
| Publisher | SCIENTIFIC REPORTS |
| Citation | SCIENTIFIC REPORTS.2017,7. |
| Abstract | Human embryonic stem cells (hESCs) are a unique population of cells defined by their capacity for self-renewal and pluripotency. Here, we identified a previously uncharacterized gene in hESCs, C9ORF135, which is sharply downregulated during gastrulation and gametogenesis, along with the pluripotency factors OCT4, SOX2, and NANOG. Human ESCs express two C9ORF135 isoforms, the longer of which encodes a membrane-associated protein, as determined by immunostaining and western blotting of fractionated cell lysates. Moreover, the results of chromatin immunoprecipitation (ChIP), mass spectrometry (MS), and co-immunoprecipitation (co-IP) analyses demonstrated that C9ORF135 expression is regulated by OCT4 and SOX2 and that C9ORF135 interacts with non-muscle myosin IIA and myosin IIB. Collectively, these data indicated that C9ORF135 encodes a membrane-associated protein that may serve as a surface marker for undifferentiated hESCs. |
| URI | http://hdl.handle.net/20.500.11897/474484 |
| ISSN | 2045-2322 |
| DOI | 10.1038/srep45311 |
| Indexed | SCI(E) |
| Appears in Collections: | 人民医院 |
