| Title | Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana |
| Authors | Chowdhary, Anuradha Hagen, Ferry Sharma, Cheshta Al-Hatmi, Abdullah M. S. Giuffre, Letterio Giosa, Domenico Fan, Shangrong Badali, Hamid Felice, Maria Rosa de Hoog, Sybren Meis, Jacques F. Romeo, Orazio |
| Affiliation | Univ Delhi, Vallabhbhai Patel Chest Inst, Dept Med Mycol, New Delhi, India. Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands. Westerdijk Fungal Biodivers Inst, Utrecht, Netherlands. Ibri Hosp, Directorate Gen Hlth Serv, Minist Hlth, Ibri, Oman. Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, Messina, Italy. Ctr Neurolesi Bonino Pulejo, Sci Inst Res Hospitalizat & Hlth Care, Messina, Italy. Peking Univ, Shenzhen Hosp, Dept Obstet & Gynecol, Shenzhen, Peoples R China. Mazandaran Univ Med Sci, Sch Med, Dept Med Mycol & Parasitol, Invas Fungi Res Ctr, Sari, Iran. Canisius Wilhelmina Ziekenhuis, Ctr Expertise Mycol Radboudumc, Nijmegen, Netherlands. Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, Messina, Italy. Romeo, O (reprint author), Ctr Neurolesi Bonino Pulejo, Sci Inst Res Hospitalizat & Hlth Care, Messina, Italy. |
| Keywords | Candida africana Candida albicans genotyping multi-locus sequence typing amplified fragment length polymorphisms VULVO-VAGINAL CANDIDIASIS MOLECULAR CHARACTERIZATION ANTIFUNGAL SUSCEPTIBILITY ALBICANS DUBLINIENSIS EPIDEMIOLOGY STRAINS STELLATOIDEA PREVALENCE INDIA |
| Issue Date | 2017 |
| Publisher | FRONTIERS IN MICROBIOLOGY |
| Citation | FRONTIERS IN MICROBIOLOGY.2017,8. |
| Abstract | This study aimed at investigating the genetic diversity of a panel of Candida africana strains recovered from vaginal samples in different countries. All fungal strains were heterozygous at the mating-type-like locus and belonged to the genotype A of Candida albicans. Moreover, all examined C. africana strains lack N-acetylglucosamine assimilation and sequence analysis of the HXK1 gene showed a distinctive polymorphism that impair the utilization of this amino sugar in this yeast. Multi-locus sequencing of seven housekeeping genes revealed a substantial genetic homogeneity among the strains, except for the CaMPIb, SYA1 and VPS13 loci which contributed significantly to the classification of our set of C. africana strains into six existing diploid sequence types. Amplified fragment length polymorphism fingerprint analysis yielded greater genotypic heterogeneity among the C. africana strains. Overall the data reported here show that in C. africana genetic diversity occurs and the existence of this intriguing group of C. albicans strains with specific phenotypes associated could be useful for future comparative studies in order to better understand the genetics and evolution of this important human pathogen. |
| URI | http://hdl.handle.net/20.500.11897/474108 |
| ISSN | 1664-302X |
| DOI | 10.3389/fmicb.2017.00556 |
| Indexed | SCI(E) |
| Appears in Collections: | 深圳医院 |
