| Title | Abnormalities of the Bone Marrow Immune Microenvironment in Patients with Prolonged Isolated Thrombocytopenia after Allogeneic Hematopoietic Stem Cell Transplantation |
| Authors | Song, Yang Shi, Min-Min Zhang, Yuan-Yuan Mo, Xiao-Dong Wang, Yu Zhang, Xiao-Hui Xu, Lan-Ping Huang, Xiao-Jun Kong, Yuan |
| Affiliation | Peking Univ, Peking Univ Peoples Hosp, Collaborat Innovat Ctr Hematol, Peking Univ Inst Hematol,Beijing Key Lab Hematopo, Beijing 100044, Peoples R China. Peking Univ, Acad Adv Interdisciplinary Studies, Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China. |
| Keywords | Prolonged isolated thrombocytopenia Bone marrow immune microenvironment Allogeneic hematopoietic stem cell transplantation POOR GRAFT FUNCTION CD8(+) T-CELLS MEGAKARYOCYTE APOPTOSIS PLATELET COUNT IN-VITRO PURPURA CYTOKINE BLOOD INTERLEUKIN-17 ASSOCIATION |
| Issue Date | 2017 |
| Publisher | BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION |
| Citation | BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION.2017,23(6),906-912. |
| Abstract | Prolonged isolated thrombocytopenia (PT) is a serious complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Whether abnormalities of the bone marrow (BM) immune microenvironment are involved in the pathogenesis of PT remains unknown, however. Twenty patients with PT, 40 matched patients with good graft function (GGF) after allo-HSCT, and 20 healthy donors (HD) were enrolled in this nested case-control study. Th1, Th2, Tc1, Tc2, Th17, and Treg cells were analyzed by flow cytometry, and IFN-gamma, IL-4, IL-17, IL-6, IL-21, and thrombopoietin levels in BM plasma were evaluated with a cytometric bead assay and ELISA. Relative to GGF patients and HD controls, PT patients had significantly higher proportions of Th1 and Tc1 cells, resulting in higher Th1/Th2 and Tc1/Tc2 ratios in the BM microenvironment. In addition, the excessive polarization of Th17 was observed in patients with PT. Changes in BM plasma cytokines were consistent with our cellular findings. These results suggest that dysregulated T cell responses in the BM microenvironment might play an important role in the pathogenesis of PT. (C) 2017 American Society for Blood and Marrow Transplantation. |
| URI | http://hdl.handle.net/20.500.11897/473113 |
| ISSN | 1083-8791 |
| DOI | 10.1016/j.bbmt.2017.02.021 |
| Indexed | SCI(E) |
| Appears in Collections: | 人民医院 前沿交叉学科研究院 |
