| Title | Influence of Freeze-Thaw Cycles on RNA Integrity of Gastrointestinal Cancer and Matched Adjacent Tissues |
| Authors | Hu, Ying Han, Haibo Wang, Yixue Song, Lijie Cheng, Xiaojing Xing, Xiaofang Dong, Bin Wang, Xiaohong Chen, Meng Zhang, Lianhai Ji, Jiafu |
| Affiliation | Peking Univ, Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res, Dept BioBank,Minist Educ, 52 Fu Cheng Rd, Beijing 100142, Peoples R China. Peking Univ, Canc Hosp & Inst, Div Gastrointestinal Canc Translat Res Lab, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing, Peoples R China. Peking Univ, Canc Hosp & Inst, Dept Pathol, Beijing, Peoples R China. Peking Univ, Canc Hosp & Inst, Dept Gastrointestinal Surg, Beijing 100142, Peoples R China. Peking Univ, Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res, Dept BioBank,Minist Educ, 52 Fu Cheng Rd, Beijing 100142, Peoples R China. Zhang, LH (reprint author), Peking Univ, Canc Hosp & Inst, Dept Gastrointestinal Surg, Beijing 100142, Peoples R China. |
| Keywords | RNA integrity number (RIN) freeze-thaw cycles QUALITY IMPACT BIOBANKING STORAGE TIME |
| Issue Date | 2017 |
| Publisher | BIOPRESERVATION AND BIOBANKING |
| Citation | BIOPRESERVATION AND BIOBANKING.2017,15(3),241-247. |
| Abstract | Background: Comparative analysis of RNA expression profiles between cancer and adjacent noncancerous tissues is an important part of cancer research. High-quality RNA is essential for consistent, reliable results, especially for identification of cancer biomarkers. However, the impact of freeze-thaw cycles on the quality of RNA both in gastrointestinal cancer and paired adjacent tissues is still unclear. Aim: To investigate the influence of freeze-thaw cycles on RNA integrity and overall histomorphology of gastrointestinal cancer and paired adjacent noncancerous tissues. Methods: Gastrointestinal cancer and matched adjacent noncancerous tissues were frozen and thawed twice before extracting RNA. Total RNA in each sample was extracted with TRIzol reagents and the RNA integrity was assessed by RNA integrity number (RIN) on an Agilent Bioanalyzer. Light microscopy was then used to assess tissue composition and morphology. Results: RIN values for all samples tended to decrease in correlation with the frequency of freeze-thawing. With an RIN cutoff value of 6, RNA extracted from pancreatic cancer tissues was not qualified after the first freeze-thaw cycle. Moreover, all RNA extracted from adjacent noncancerous tissues had nonqualifying RIN scores after the first freeze-thaw cycle, except for liver tissues. Microscopically, all samples displayed qualified tissue morphology regardless of freeze-thaw cycle frequency. Conclusion: Freeze-thawing affects the RNA integrity, but not the tissue morphology of gastrointestinal cancer and paired adjacent noncancerous tissues. Furthermore, the RNA extracted from adjacent noncancerous tissues is more easily degraded than that in cancer tissues. |
| URI | http://hdl.handle.net/20.500.11897/473084 |
| ISSN | 1947-5535 |
| DOI | 10.1089/bio.2016.0035 |
| Indexed | SCI(E) |
| Appears in Collections: | 北京肿瘤医院 |
