| Title | High Prevalence of Macrolide-resistance and Molecular Characterization of Streptococcus pyogenes Isolates Circulatingin China from 2009 to 2016 |
| Authors | Lu, Binghuai Fang, Yujie Fan, Yanyan Chen, Xingchun Wang, Junrui Zeng, Ji Li, Yi Zhang, Zhijun Huang, Lei Li, Hongxia Li, Dong Zhu, Fengxia Cui, Yanchao Wang, Duochun |
| Affiliation | Peking Univ, Civil Aviat Sch Clin Med, Civil Aviat Gen Hosp, Dept Lab Med, Beijing, Peoples R China. Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China. Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China. China Japan Friendship Hosp, Dept Pulm & Crit Care Med, Lab Clin Microbiol & Infect Dis, Beijing, Peoples R China. Peoples Hosp Guangxi Zhuang Autonomous Reg, Dept Lab Med, Nanning, Peoples R China. Inner Mongolia Med Univ, Affiliated Hosp, Dept Clin Lab, Hohhot, Peoples R China. Univ Sci & Technol, Wuhan Pu Ai Hosp Huazhong, Dept Lab Med, Wuhan, Peoples R China. Henan Prov Peoples Hosp, Dept Lab Med, Zhengzhou, Peoples R China. Taian City Cent Hosp Taian, Dept Lab Med, Tai An, Shandong, Peoples R China. Peking Univ, Hosp 1, Dept Lab Med, Beijing, Peoples R China. Chengdu First Peoples Hosp Chengdu, Dept Lab Med, Chengdu, Sichuan, Peoples R China. Peking Univ, Civil Aviat Sch Clin Med, Civil Aviat Gen Hosp, Dept Lab Med, Beijing, Peoples R China. Wang, DC (reprint author), Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China. Wang, DC (reprint author), Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China. |
| Keywords | molecular characterization antibiotic resistance Streptococcus pyogenes EMM-TYPES FLUOROQUINOLONE RESISTANCE VACCINE DEVELOPMENT 7-YEAR PERIOD CHILDREN EQUISIMILIS INFECTIONS EMERGENCE STRAINS DISEASE |
| Issue Date | 2017 |
| Publisher | FRONTIERS IN MICROBIOLOGY |
| Citation | FRONTIERS IN MICROBIOLOGY.2017,8. |
| Abstract | Streptococcus pyogenes, or group A Streptococcus, is a pathogen responsible for a wide range of clinical manifestations, from mild skin and soft tissue infections and pharyngitis to severe diseases. Its epidemiological characteristics should be comprehensively under surveillance for regulating the national prevention and treatment practice. Herein, a total of 140 S. pyogenes, including 38 invasive and 102 noninvasive isolates, were collected from infected patients in 10 tertiary general hospitals from 7 cities/provinces in China during the years 2009-2016. All strains were characterized by classical and molecular techniques for its emm types/subtypes, virulent factors and antibiotic resistance profiling. Of 140 isolates, 15 distinct emm types and 31 subtypes were detected, dominated by emm12 (60 isolates, 42.9%), emm1(43, 30.7%), and emm89 (10, 7.1%), and 8 new emm variant subtypes were identified. All strains, invasive or not, harbored the superantigenic genes, speB and slo. The other virulence genes, smeZ, speF, and speC accounted for 96.4, 91.4, and 87.1% of collected isolates, respectively. Furthermultilocus sequence typing (MLST) placed all strains into 22 individual sequence types (STs), including 4 newly-identified STs (11, 7.9%). All isolates were phenotypically susceptible to penicillin, ampicillin, cefotaxime, and vancomycin, whereas 131(93.5%), 132(94.2%), and 121(86.4%) were resistant to erythromycin, clindamycin, and tetracycline, respectively. Our study highlights high genotypic diversity and high prevalence of macrolide resistance of S. pyogenes among clinical isolates circulating in China. |
| URI | http://hdl.handle.net/20.500.11897/472857 |
| ISSN | 1664-302X |
| DOI | 10.3389/fmicb.2017.01052 |
| Indexed | SCI(E) |
| Appears in Collections: | 北京民用航空总医院 第一医院 |
