| Title | Astrocyte activation and capillary remodeling in modified bilateral common carotid artery occlusion mice |
| Authors | Song, Jiangman Nan, Di He, Qihua Yang, Lu Guo, Huailian |
| Affiliation | Peking Univ, Dept Neurol, Peoples Hosp, Beijing, Peoples R China. Peking Univ, Ctr Med & Hlth Anal, Beijing, Peoples R China. |
| Keywords | astrocyte activation capillary remodeling cerebral ischemia in vivo modified BCCAO model CEREBRAL HYPOPERFUSION REACTIVE ASTROCYTES ISCHEMIC-STROKE CHRONIC HYPOXIA MICROVASCULATURE NEUROPROTECTION ANGIOGENESIS DENSITY MODEL |
| Issue Date | 2017 |
| Publisher | MICROCIRCULATION |
| Citation | MICROCIRCULATION.2017,24(6). |
| Abstract | ObjectiveThe cerebral ischemia leads to brain dysfunction with neuron degeneration and responses from astrocytes and vessels. The aim of this study was to study the changes of astrocyte and microvessel in modified BCCAO mice. MethodsAdult transgenic Tie2-GFP mice were subjected to modified BCCAO operation and cranial window implantation. CBF and neurological injury were examined after ischemia. Astrocytes and vessels were investigated by two-photon laser-scanning microscope and confocal laser-scanning microscope in vivo. ResultsThe CBF decreased to approximately 40% of the baseline in the ischemic mice (P<.05). The neuron damage was explicit after the cerebral ischemia (P<.05), while no significant impairment of the motor and cognitive function was detected (P>.05). The density of astrocyte and volume of the astrocyte soma was increased significantly after ischemia (P<.01). Meanwhile, the mean distance between the penetrating artery and the nearest astrocyte soma decreased significantly (P<.01). Besides, the increased diameter of capillary and change of vessel arrangement were observed. ConclusionThe cerebral ischemia was successfully induced by this modified BCCAO model. Astrocyte activation and the capillary remodeling, including dilution of capillary and tortuosity, were observed in this model. |
| URI | http://hdl.handle.net/20.500.11897/471740 |
| ISSN | 1073-9688 |
| DOI | 10.1111/micc.12366 |
| Indexed | SCI(E) |
| Appears in Collections: | 人民医院 |
