Title | Different susceptibility and pathogenesis of rabbit genotype 3 hepatitis E virus (HEV-3) and human HEV-3 (JRC-HE3) in SPF rabbits |
Authors | Zhang, Yulin Gong, Wanyun Song, William Tianshi Fu, Hongwei Wang, Lin Li, Manyu Wang, Ling Zhuang, Hui |
Affiliation | Peking Univ, Hlth Sci Ctr, Ctr Infect Dis, Dept Microbiol, Beijing 100191, Peoples R China. Peking Univ, Hlth Sci Ctr, Ctr Infect Dis, Sch Basic Med Sci, Beijing 100191, Peoples R China. Tianjin Med Univ, Sch Lab Med, Tianjin 300203, Peoples R China. Peking Univ, Hlth Sci Ctr, Ctr Infect Dis, Dept Microbiol, Beijing 100191, Peoples R China. Wang, L (reprint author), Peking Univ, Hlth Sci Ctr, Ctr Infect Dis, Sch Basic Med Sci, Beijing 100191, Peoples R China. |
Keywords | HEV genotype 3 (HEV-3) Infection Pathogenesis Rabbits CROSS-SPECIES INFECTION STRAIN CHINA SWINE PIGS TRANSMISSION COUNTRIES ANIMALS DISEASE KIDNEY |
Issue Date | 2017 |
Publisher | VETERINARY MICROBIOLOGY |
Citation | VETERINARY MICROBIOLOGY.2017,207,1-6. |
Abstract | Hepatitis E virus (HEV) is an increasingly important zoonotic infection in humans with HEV genotypes 3 and 4 being recognized as zoonotic pathogens. The relatively recently isolated genotype 3 rabbit HEV (rHEV-3) and the more well known genotype 3 isolates from humans and swine (hsHEV-3) have all been confirmed experimentally to be capable of infecting both non-human primates and specific-pathogen free (SPF) pigs. In a previous study rHEV-3 was shown to cause acute hepatitis in experimentally infected rabbits. However, whether hsHEV-3 can productively infect rabbits remained unclear. The objective of this study was to investigate the experimental infection of rabbits with human HEV-3 (hHEV-3, JRC-HE3), to compare it to that with rHEV-3 (CHN-BJ-rb14) and to further characterise the pathogenesis of the two isolates. All animals inoculated with rHEV-3 (CHN-BJ-rb14) became infected, exhibiting an intermittent viremia, elevated liver enzymes, and persistent fecal virus shedding throughout the 15 week study period. Liver histopathology showed acute inflammation and both positive- and negative-stranded viral RNA was detected in various tissues from necropsied rabbits. By contrast, neither sero-conversion nor alanine aminotransferase (ALT) elevation was observed in most rabbits inoculated with hHEV-3 (JRC-HE3). In addition, rHEV-3 (CHN-BJ-rb14) but not hHEV-3 (JRC-HE3) recovered from primary infected rabbits was transmissible to naive rabbits. These results showed that SPF rabbits are readily susceptible to infection with rHEV-3 (CHN-BJ-rb14) but not hHEV-3 (JRC-HE3), which might indicate the influence of viral genomic organization on its pathogenicity. |
URI | http://hdl.handle.net/20.500.11897/471725 |
ISSN | 0378-1135 |
DOI | 10.1016/j.vetmic.2017.05.019 |
Indexed | SCI(E) |
Appears in Collections: | 医学部待认领 |