| Title | Characteristics of Labial Gland Mesenchymal Stem Cells of Healthy Individuals and Patients with Sjogren's Syndrome: A Preliminary Study |
| Authors | Wang, Shi-Qin Wang, Yi-Xiang Hua, Hong |
| Affiliation | Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Dept Oral Med, 22 South Zhongguancun Ave, Beijing 100081, Peoples R China. Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Dept Oral Surg, 22 South Zhongguancun Ave, Beijing 100081, Peoples R China. Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Dept Oral Med, 22 South Zhongguancun Ave, Beijing 100081, Peoples R China. Wang, YX (reprint author), Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Dept Oral Surg, 22 South Zhongguancun Ave, Beijing 100081, Peoples R China. |
| Keywords | mesenchymal stem cells Sjogren's syndrome salivary glands labial glands HUMAN SALIVARY-GLANDS BONE-MARROW STEM/PROGENITOR CELLS TISSUE DIFFERENTIATION LOCALIZATION ACTIVATION EXPRESSION THERAPY |
| Issue Date | 2017 |
| Publisher | STEM CELLS AND DEVELOPMENT |
| Citation | STEM CELLS AND DEVELOPMENT.2017,26(16),1171-1185. |
| Abstract | Sjogren's syndrome (SS) is a systemic autoimmune disease that is characterized by focal lymphocytic infiltration into exocrine organs such as salivary and lacrimal glands, resulting in dry mouth and eyes, and other systemic injuries. There is no curative clinical therapy for SS, and stem cell therapy has shown great potential in this area. The mesenchymal stem cells (MSCs) in the salivary glands of healthy individuals and in patients with SS have not been extensively studied. The aim of this study was to elucidate the characteristics of MSCs from the labial glands of healthy controls and of those from patients with SS to elucidate the related pathogenesis and to uncover potential avenues for novel clinical interventions. Labial glands from patients with SS and healthy subjects were obtained, and MSCs were isolated and cultured by using the tissue adherent method. The MSC characteristics of the cultured cells were confirmed by using morphology, proliferation, colony forming-unit (CFU) efficiency, and multipotentiality, including osteogenic, adipogenic, and salivary gland differentiation. The MSCs from the healthy controls and SS patients expressed characteristic MSC markers, including CD29, CD44, CD73, CD90, and CD105; they were negative for CD34, CD45, and CD106, and also negative for the salivary gland epithelium markers (CD49f and CD117). Labial gland MSCs from both groups were capable of osteogenic and adipogenic differentiation. The CFU efficiency and adipogenic differentiation potential of MSCs were significantly lower in the SS group compared with the healthy controls. Cells from both groups could also be induced into salivary gland-like cells. Real-time polymerase chain reaction and immunofluorescence staining showed that the gene and protein expression of AMY1, AQP5, and ZO-1 in cells from the SS group was lower than that in cells from the healthy group. Thus, MSCs from the labial glands in patients with SS could lack certain characteristics and functions, especially related to salivary secretion. These preliminary data provided insights that could lead to the development of novel therapeutic strategies for the treatment of SS. |
| URI | http://hdl.handle.net/20.500.11897/471472 |
| ISSN | 1547-3287 |
| DOI | 10.1089/scd.2017.0045 |
| Indexed | SCI(E) |
| Appears in Collections: | 口腔医院 |
